Mouse Anti-PAM Recombinant Antibody (CBYC-P153) (CBMAB-P0720-YC)

Name: Mouse Anti-PAM Recombinant Antibody (CBYC-P153)
SKU: CBMAB-P0720-YC
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBYC-P153

Application

WB, ICC, IHC-P, IHC-Fr, ELISA

Immunogen

Peptidylglycine Alpha Amidating Monooxygenase

Specificity

Human

Antibody Isotype

IgG

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

PBS, pH 7.4, 0.02% sodium azide, 50% glycerol

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Peptidylglycine Alpha-Amidating Monooxygenase

Introduction

PAM is a multifunctional protein. The encoded preproprotein is proteolytically processed to generate the mature enzyme. This enzyme includes two domains with distinct catalytic activities, a peptidylglycine alpha-hydroxylating monooxygenase (PHM) domain and a peptidyl-alpha-hydroxyglycine alpha-amidating lyase (PAL) domain. These catalytic domains work sequentially to catalyze the conversion of neuroendocrine peptides to active alpha-amidated products. Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed.

Entrez Gene ID

5066

UniProt ID

P19021

Alternative Names

PAL; PHM

Function

Bifunctional enzyme that catalyzes the post-translational modification of inactive peptidylglycine precursors to the corresponding bioactive alpha-amidated peptides, a terminal modification in biosynthesis of many neural and endocrine peptides (PubMed:12699694).
Alpha-amidation involves two sequential reactions, both of which are catalyzed by separate catalytic domains of the enzyme. The first step, catalyzed by peptidyl alpha-hydroxylating monooxygenase (PHM) domain, is the copper-, ascorbate-, and O2- dependent stereospecific hydroxylation (with S stereochemistry) at the alpha-carbon (C-alpha) of the C-terminal glycine of the peptidylglycine substrate (PubMed:12699694).
The second step, catalyzed by the peptidylglycine amidoglycolate lyase (PAL) domain, is the zinc-dependent cleavage of the N-C-alpha bond, producing the alpha-amidated peptide and glyoxylate (PubMed:12699694).
Similarly, catalyzes the two-step conversion of an N-fatty acylglycine to a primary fatty acid amide and glyoxylate (By similarity).

Biological Process

Central nervous system developmentIEA:Ensembl
Fatty acid primary amide biosynthetic processISS:UniProtKB
Heart developmentIEA:Ensembl
LactationIEA:Ensembl
Limb developmentIEA:Ensembl
Long-chain fatty acid metabolic processIEA:Ensembl
Maternal process involved in female pregnancyIEA:Ensembl
OdontogenesisIEA:Ensembl
Ovulation cycle processIEA:Ensembl
Peptide amidationManual Assertion Based On ExperimentIDA:UniProtKB
Protein amidationIEA:Ensembl
Regulation of actin cytoskeleton organizationIEA:Ensembl
Regulation of protein secretionIEA:Ensembl
Regulation of transcription by RNA polymerase IIIEA:Ensembl
Response to copper ionIEA:Ensembl
Response to estradiolIEA:Ensembl
Response to glucocorticoidIEA:Ensembl
Response to hypoxiaIEA:Ensembl
Response to pHIEA:Ensembl
Response to xenobiotic stimulusIEA:Ensembl
Response to zinc ionManual Assertion Based On ExperimentIDA:UniProtKB
Toxin metabolic processIEA:Ensembl

Cellular Location

Cytoplasmic vesicle, secretory vesicle membrane
Secretory granules.
Isoform 1
Membrane
Isoform 2
Membrane
Isoform 3
Secreted
Secreted from secretory granules.
Isoform 4
Secreted
Secreted from secretory granules.

Topology

Intragranular: 31-863
Helical: 864-887
Cytoplasmic: 888-973

Ilina, Y., Kaufmann, P., Melander, O., Press, M., Thuene, K., & Bergmann, A. (2023). Immunoassay-based quantification of full-length peptidylglycine alpha-amidating monooxygenase in human plasma. Scientific Reports, 13(1), 10827.

Umapathysivam, M. M., Araldi, E., Hastoy, B., Dawed, A. Y., Vatandaslar, H., Sengupta, S., ... & Gloyn, A. L. (2023). Type 2 diabetes risk alleles in peptidyl-glycine alpha-amidating monooxygenase influence GLP-1 levels and response to GLP-1 receptor agonists. medRxiv.

Morrison, D. G. (2022). Heterologous production of recombinant peptidylglycine α-amidating monooxygenase for the production of biosimilar α-amidated peptides.

Sim, J., Marginean, H., Jirovec, A., Vickers, M. M., Marginean, E. C., Asmis, T. R., ... & Goodwin, R. A. (2022). 895P Correlating peptidylglycine alpha-amidating monooxygenase (PAM) expression with clinicopathologic variables in gastrointestinal (GI) neuroendocrine tumors (NETs). Annals of Oncology, 33, S957.

Merkler, D., Hawley, A., Eipper, B., & Mains, R. (2021). Peptidylglycine α-amidating monooxygenase as a therapeutic target or biomarker. Authorea Preprints.

Mamoor, S. (2020). The peptidylglycine alpha-amidating monooxygenase, PAM, is differentially expressed in viral co-infections.

Bäck, N., Luxmi, R., Powers, K. G., Mains, R. E., & Eipper, B. A. (2020). Peptidylglycine α-amidating monooxygenase is required for atrial secretory granule formation. Proceedings of the National Academy of Sciences, 117(30), 17820-17831.

Donlon, J., & Ryan, P. (2019). Peptidylglycine monooxygenase activity of monomeric species of growth hormone. Heliyon, 5(9).

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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