Human Recombinant PAM protein, His Tag (V2LY-0526-LY5960)

Name: Human Recombinant PAM protein, His Tag
SKU: V2LY-0526-LY5960
Rating: 5 (1 reviews)

See all PAM antibodies

Compare Online Inquiry

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Human

Tag

His Tag

Protein Construction

This product is Human Recombinant PAM protein, His Tag consist of Amino Acid: 1-710 and predicts a molecular mass of 78.68 kDa.

Molecule Mass

78.68 kDa

Verified

HPLC

Sequence

Amino Acid: 1-710

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

≥90% as determined by SDS-PAGE. ≥90% as determined by SEC-HPLC.

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile PBS

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

Peptidylglycine Alpha-Amidating Monooxygenase

Function

Bifunctional enzyme that catalyzes the post-translational modification of inactive peptidylglycine precursors to the corresponding bioactive alpha-amidated peptides, a terminal modification in biosynthesis of many neural and endocrine peptides (PubMed:12699694).
Alpha-amidation involves two sequential reactions, both of which are catalyzed by separate catalytic domains of the enzyme. The first step, catalyzed by peptidyl alpha-hydroxylating monooxygenase (PHM) domain, is the copper-, ascorbate-, and O2- dependent stereospecific hydroxylation (with S stereochemistry) at the alpha-carbon (C-alpha) of the C-terminal glycine of the peptidylglycine substrate (PubMed:12699694).
The second step, catalyzed by the peptidylglycine amidoglycolate lyase (PAL) domain, is the zinc-dependent cleavage of the N-C-alpha bond, producing the alpha-amidated peptide and glyoxylate (PubMed:12699694).
Similarly, catalyzes the two-step conversion of an N-fatty acylglycine to a primary fatty acid amide and glyoxylate (By similarity).

Biological Process

Central nervous system developmentIEA:Ensembl
Fatty acid primary amide biosynthetic processISS:UniProtKB
Heart developmentIEA:Ensembl
LactationIEA:Ensembl
Limb developmentIEA:Ensembl
Long-chain fatty acid metabolic processIEA:Ensembl
Maternal process involved in female pregnancyIEA:Ensembl
OdontogenesisIEA:Ensembl
Ovulation cycle processIEA:Ensembl
Peptide amidationManual Assertion Based On ExperimentIDA:UniProtKB
Protein amidationIEA:Ensembl
Regulation of actin cytoskeleton organizationIEA:Ensembl
Regulation of protein secretionIEA:Ensembl
Regulation of transcription by RNA polymerase IIIEA:Ensembl
Response to copper ionIEA:Ensembl
Response to estradiolIEA:Ensembl
Response to glucocorticoidIEA:Ensembl
Response to hypoxiaIEA:Ensembl
Response to pHIEA:Ensembl
Response to xenobiotic stimulusIEA:Ensembl
Response to zinc ionManual Assertion Based On ExperimentIDA:UniProtKB
Toxin metabolic processIEA:Ensembl

Cellular Location

Cytoplasmic vesicle, secretory vesicle membrane
Secretory granules.
Isoform 1
Membrane
Isoform 2
Membrane
Isoform 3
Secreted
Secreted from secretory granules.
Isoform 4
Secreted
Secreted from secretory granules.

Topology

Intragranular: 31-863
Helical: 864-887
Cytoplasmic: 888-973

Ilina, Y., Kaufmann, P., Melander, O., Press, M., Thuene, K., & Bergmann, A. (2023). Immunoassay-based quantification of full-length peptidylglycine alpha-amidating monooxygenase in human plasma. Scientific Reports, 13(1), 10827.

Umapathysivam, M. M., Araldi, E., Hastoy, B., Dawed, A. Y., Vatandaslar, H., Sengupta, S., ... & Gloyn, A. L. (2023). Type 2 diabetes risk alleles in peptidyl-glycine alpha-amidating monooxygenase influence GLP-1 levels and response to GLP-1 receptor agonists. medRxiv.

Morrison, D. G. (2022). Heterologous production of recombinant peptidylglycine α-amidating monooxygenase for the production of biosimilar α-amidated peptides.

Sim, J., Marginean, H., Jirovec, A., Vickers, M. M., Marginean, E. C., Asmis, T. R., ... & Goodwin, R. A. (2022). 895P Correlating peptidylglycine alpha-amidating monooxygenase (PAM) expression with clinicopathologic variables in gastrointestinal (GI) neuroendocrine tumors (NETs). Annals of Oncology, 33, S957.

Merkler, D., Hawley, A., Eipper, B., & Mains, R. (2021). Peptidylglycine α-amidating monooxygenase as a therapeutic target or biomarker. Authorea Preprints.

Mamoor, S. (2020). The peptidylglycine alpha-amidating monooxygenase, PAM, is differentially expressed in viral co-infections.

Bäck, N., Luxmi, R., Powers, K. G., Mains, R. E., & Eipper, B. A. (2020). Peptidylglycine α-amidating monooxygenase is required for atrial secretory granule formation. Proceedings of the National Academy of Sciences, 117(30), 17820-17831.

Donlon, J., & Ryan, P. (2019). Peptidylglycine monooxygenase activity of monomeric species of growth hormone. Heliyon, 5(9).

Ask a question We look forward to hearing from you.

0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Have you used Human Recombinant PAM protein, His Tag?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon

$30 eGift Card
Submit a review

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human Recombinant PAM protein, hFc Tag (CAT#: V2LY-0526-LY5959)

Related Products

Mouse Anti-PAM Recombinant Antibody (F-4) (CAT#: CBMAB-P0718-YC)

Rabbit Anti-PAM Recombinant Antibody (CBYC-P151) (CAT#: CBMAB-P0717-YC)

Human Recombinant PAM protein, hFc Tag (CAT#: V2LY-0526-LY5959)

Mouse Anti-PAM Recombinant Antibody (NA) (CAT#: CBMAB-AO861LY)

Mouse Anti-PAM Recombinant Antibody (CBYC-P153) (CAT#: CBMAB-P0720-YC)

Mouse Anti-PAM Recombinant Antibody (CBYC-P152) (CAT#: CBMAB-P0719-YC)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Request bulk or custom quote Second antibody and isotype control

Contact us

Tel: (USA)
(UK)
Fax:

Global Locations

Email:

Online Inquiry