Rabbit Anti-PANX1 Recombinant Antibody (
28G5) (V2LY-0725-LY1229)

Name: Rabbit Anti-PANX1 Recombinant Antibody (28G5)
SKU: V2LY-0725-LY1229
Rating: 5 (1 reviews)

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Tested Data

Rabbit Anti-PANX1 Recombinant Antibody (28G5) (V2LY-0725-LY1229) in IHC

IHC image of V2LY-0725-LY1229 diluted at 1:50 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.1:50% DAB.

Rabbit Anti-PANX1 Recombinant Antibody (28G5) (V2LY-0725-LY1229) in IHC

IHC image of V2LY-0725-LY1229 diluted at 1:50 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.1:50% DAB.

Rabbit Anti-PANX1 Recombinant Antibody (28G5) (V2LY-0725-LY1229) in IF

Immunofluorescence staining of U251 with V2LY-0725-LY1229 at 1:10, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 502-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Rabbit Anti-PANX1 Recombinant Antibody (28G5) (V2LY-0725-LY1229) in FC

Overlay Peak curve showing A549 cells surface stained with V2LY-0725-LY1229 (red line) at 1:50. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1µg/1*106cells) for 45min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4°C.Control antibody (green line) was rabbit IgG (1µg/1*106cells) used under the same conditions. Acquisition of>10,000 events was performed.

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Rabbit

Clone

28G5

Application

ELISA, IHC, IF, FC

Immunogen

A synthesized peptide derived from Human PANX1.

Host Species

Rabbit

Specificity

Human

Antibody Isotype

IgG

Clonality

Monoclonal Antibody

Application Notes

Application	Note
IHC	1:50-1:200
IF	1:50-1:200
FC	1:50-1:200

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, glycerol

Preservative

Sodium azide

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Pannexin 1

Entrez Gene ID

24145

UniProt ID

Q96RD7

Function

Structural component of the gap junctions and the hemichannels involved in the ATP release and nucleotide permeation (PubMed:16908669, PubMed:20829356, PubMed:30918116).
May play a role as a Ca2+-leak channel to regulate ER Ca2+ homeostasis (PubMed:16908669).
Plays a critical role in oogenesis (PubMed:30918116).

Biological Process

Calcium ion transportManual Assertion Based On ExperimentIMP:UniProtKB
Cation transportManual Assertion Based On ExperimentIDA:BHF-UCL
Cell-cell signalingManual Assertion Based On ExperimentIBA:GO_Central
OogenesisIEA:UniProtKB-KW
Positive regulation of interleukin-1 alpha productionIEA:Ensembl
Positive regulation of interleukin-1 beta productionManual Assertion Based On ExperimentIDA:BHF-UCL
Response to ATPIEA:Ensembl
Response to ischemiaIEA:Ensembl

Cellular Location

Cell membrane
Cell junction, gap junction
Endoplasmic reticulum membrane

Involvement in disease

Oocyte maturation defect 7 (OOMD7):
An autosomal dominant infertility disorder due to oocyte degeneration and death, which may occur before or after fertilization.

Topology

Cytoplasmic: 1-40
Helical: 41-61
Extracellular: 62-106
Helical: 107-127
Cytoplasmic: 128-217
Helical: 218-238
Extracellular: 239-266
Helical: 267-287
Cytoplasmic: 288-426

PTM

S-nitrosylation inhibits channel currents and ATP release.
N-glycosylation may play a role in cell surface targeting (By similarity).
Exists in three glycosylation states: non-glycosylated (GLY0), high-mannose glycosylated (GLY1), and fully mature glycosylated (GLY2) (PubMed:30918116).

Yang, K., Xiao, Z., He, X., Weng, R., Zhao, X., & Sun, T. (2022). Mechanisms of pannexin 1 (PANX1) channel mechanosensitivity and its pathological roles. International Journal of Molecular Sciences, 23(3), 1523.

Crocetti, L., Guerrini, G., Giovannoni, M. P., Melani, F., Lamanna, S., Di Cesare Mannelli, L., ... & Dahl, G. (2022). New panx-1 blockers: Synthesis, biological evaluation and molecular dynamic studies. International journal of molecular sciences, 23(9), 4827.

Bisht, K., Okojie, K. A., Sharma, K., Lentferink, D. H., Sun, Y. Y., Chen, H. R., ... & Eyo, U. B. (2021). Capillary-associated microglia regulate vascular structure and function through PANX1-P2RY12 coupling in mice. Nature communications, 12(1), 5289.

Bao, L., Sun, K., & Zhang, X. (2021). PANX1 is a potential prognostic biomarker associated with immune infiltration in pancreatic adenocarcinoma: A pan-cancer analysis. Channels, 15(1), 680-696.

Wang, W., Qu, R., Dou, Q., Wu, F., Wang, W., Chen, B., ... & Sang, Q. (2021). Homozygous variants in PANX1 cause human oocyte death and female infertility. European Journal of Human Genetics, 29(9), 1396-1404.

Crocetti, L., Guerrini, G., Puglioli, S., Giovannoni, M. P., Mannelli, L. D. C., Lucarini, E., ... & Dahl, G. (2021). Design and synthesis of the first indole-based blockers of Panx-1 channel. European journal of medicinal chemistry, 223, 113650.

Molica, F., Meens, M. J., Pelli, G., Hautefort, A., Emre, Y., Imhof, B. A., ... & Kwak, B. R. (2019). Selective inhibition of Panx1 channels decreases hemostasis and thrombosis in vivo. Thrombosis research, 183, 56-62.

Shi, G., Liu, C., Yang, Y., Song, L., Liu, X., Wang, C., ... & Zhong, L. (2019). Panx1 promotes invasion-metastasis cascade in hepatocellular carcinoma. Journal of Cancer, 10(23), 5681.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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