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Mouse Anti-PARG Recombinant Antibody (1B5) (CBMAB-P0776-YC)

Provided herein is a Mouse monoclonal antibody against Human Poly(ADP-Ribose) Glycohydrolase. The antibody can be used for immunoassay techniques, such as WB, IHC.
See all PARG antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
1B5
Antibody Isotype
IgG1
Application
WB, IHC

Basic Information

Immunogen
Human recombinant protein fragment corresponding to amino acids 1-367 of human PARG(NP_003622) produced in E. coli
Specificity
Human
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
PBS, pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Poly(ADP-Ribose) Glycohydrolase
Introduction
Poly(ADP-ribose) glycohydrolase (PARG) is the major enzyme responsible for the catabolism of poly(ADP-ribose), a reversible covalent-modifier of chromosomal proteins. The protein is found in many tissues and may be subject to proteolysis generating smaller, active products.
Entrez Gene ID
8505
UniProt ID
Q86W56
Alternative Names
Poly(ADP-Ribose) Glycohydrolase; EC 3.2.1.143; Poly(ADP-Ribose) Glycohydrolase 60 KDa Isoform; Mitochondrial Poly(ADP-Ribose) Glycohydrolase; PARG99;
Function
Poly(ADP-ribose) glycohydrolase that degrades poly(ADP-ribose) by hydrolyzing the ribose-ribose bonds present in poly(ADP-ribose) (PubMed:15450800, PubMed:21892188, PubMed:23102699, PubMed:23474714, PubMed:33186521, PubMed:34321462, PubMed:34019811).
PARG acts both as an endo- and exoglycosidase, releasing poly(ADP-ribose) of different length as well as ADP-ribose monomers (PubMed:23102699, PubMed:23481255).
It is however unable to cleave the ester bond between the terminal ADP-ribose and ADP-ribosylated residues, leaving proteins that are mono-ADP-ribosylated (PubMed:21892188, PubMed:23474714, PubMed:33186521).
Poly(ADP-ribose) is synthesized after DNA damage is only present transiently and is rapidly degraded by PARG (PubMed:23102699, PubMed:34019811).
Required to prevent detrimental accumulation of poly(ADP-ribose) upon prolonged replicative stress, while it is not required for recovery from transient replicative stress (PubMed:24906880).
Responsible for the prevalence of mono-ADP-ribosylated proteins in cells, thanks to its ability to degrade poly(ADP-ribose) without cleaving the terminal protein-ribose bond (PubMed:33186521).
Required for retinoid acid-dependent gene transactivation, probably by removing poly(ADP-ribose) from histone demethylase KDM4D, allowing chromatin derepression at RAR-dependent gene promoters (PubMed:23102699).
Involved in the synthesis of ATP in the nucleus, together with PARP1, NMNAT1 and NUDT5 (PubMed:27257257).
Nuclear ATP generation is required for extensive chromatin remodeling events that are energy-consuming (PubMed:27257257).
Biological Process
ATP generation from poly-ADP-D-riboseManual Assertion Based On ExperimentIDA:UniProtKB
Base-excision repair, gap-fillingTAS:Reactome
Carbohydrate metabolic processIEA:InterPro
Nucleotide-sugar metabolic processManual Assertion Based On ExperimentIBA:GO_Central
Regulation of DNA repairManual Assertion Based On ExperimentIBA:GO_Central
Regulation of histone modificationManual Assertion Based On ExperimentIBA:GO_Central
Cellular Location
Isoform 1
Nucleus
Colocalizes with PCNA at replication foci (PubMed:21398629).
Relocalizes to the cytoplasm in response to DNA damage (PubMed:16460818).
Isoform 2
Cytoplasm
Translocates to the nucleus in response to DNA damage.
Isoform 3
Cytoplasm
Isoform 4
Cytoplasm
Mitochondrion
Isoform 5
Mitochondrion matrix
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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