Mouse Anti-PARG Recombinant Antibody (6F4) (CBMAB-P0777-YC)

Poly(ADP-Ribose) Glycohydrolase

Poly(ADP-ribose) glycohydrolase (PARG) is the major enzyme responsible for the catabolism of poly(ADP-ribose), a reversible covalent-modifier of chromosomal proteins. The protein is found in many tissues and may be subject to proteolysis generating smaller, active products.

Poly(ADP-Ribose) Glycohydrolase; EC 3.2.1.143; Poly(ADP-Ribose) Glycohydrolase 60 KDa Isoform; Mitochondrial Poly(ADP-Ribose) Glycohydrolase; PARG99;

Poly(ADP-ribose) glycohydrolase that degrades poly(ADP-ribose) by hydrolyzing the ribose-ribose bonds present in poly(ADP-ribose) (PubMed:15450800, PubMed:21892188, PubMed:23102699, PubMed:23474714, PubMed:33186521, PubMed:34321462, PubMed:34019811).
PARG acts both as an endo- and exoglycosidase, releasing poly(ADP-ribose) of different length as well as ADP-ribose monomers (PubMed:23102699, PubMed:23481255).
It is however unable to cleave the ester bond between the terminal ADP-ribose and ADP-ribosylated residues, leaving proteins that are mono-ADP-ribosylated (PubMed:21892188, PubMed:23474714, PubMed:33186521).
Poly(ADP-ribose) is synthesized after DNA damage is only present transiently and is rapidly degraded by PARG (PubMed:23102699, PubMed:34019811).
Required to prevent detrimental accumulation of poly(ADP-ribose) upon prolonged replicative stress, while it is not required for recovery from transient replicative stress (PubMed:24906880).
Responsible for the prevalence of mono-ADP-ribosylated proteins in cells, thanks to its ability to degrade poly(ADP-ribose) without cleaving the terminal protein-ribose bond (PubMed:33186521).
Required for retinoid acid-dependent gene transactivation, probably by removing poly(ADP-ribose) from histone demethylase KDM4D, allowing chromatin derepression at RAR-dependent gene promoters (PubMed:23102699).
Involved in the synthesis of ATP in the nucleus, together with PARP1, NMNAT1 and NUDT5 (PubMed:27257257).
Nuclear ATP generation is required for extensive chromatin remodeling events that are energy-consuming (PubMed:27257257).

ATP generation from poly-ADP-D-riboseManual Assertion Based On ExperimentIDA:UniProtKB
Base-excision repair, gap-fillingTAS:Reactome
Carbohydrate metabolic processIEA:InterPro
Nucleotide-sugar metabolic processManual Assertion Based On ExperimentIBA:GO_Central
Regulation of DNA repairManual Assertion Based On ExperimentIBA:GO_Central
Regulation of histone modificationManual Assertion Based On ExperimentIBA:GO_Central

Isoform 1
Nucleus
Colocalizes with PCNA at replication foci (PubMed:21398629).
Relocalizes to the cytoplasm in response to DNA damage (PubMed:16460818).
Isoform 2
Cytoplasm
Translocates to the nucleus in response to DNA damage.
Isoform 3
Cytoplasm
Isoform 4
Cytoplasm
Mitochondrion
Isoform 5
Mitochondrion matrix