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Mouse Anti-PARL Recombinant Antibody (4F4) (CBMAB-P0790-YC)

Provided herein is a Mouse monoclonal antibody against Human Presenilin Associated Rhomboid Like. The antibody can be used for immunoassay techniques, such as ELISA.
See all PARL antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
4F4
Antibody Isotype
IgG2b, κ
Application
ELISA

Basic Information

Immunogen
PSARL (NP_061092.3, 2-100 aa) partial recombinant protein with GST tag. Immunogen sequence: AWRGWAQRGW GCGQAWGASV GGRSCEELTA VLTPPQLLGR RFNFFIQQKC GFRKAPRKVE PRRSDPGTSG EAYKRSALIP PVEETVFYPS PYPIRSLIK
Specificity
Human
Antibody Isotype
IgG2b, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.
Epitope
aa 2-100

Target

Full Name
Presenilin Associated Rhomboid Like
Introduction
PARL is a member of the rhomboid family of intramembrane serine proteases that is localized to the inner mitochondrial membrane. The encoded protein regulates mitochondrial remodeling and apoptosis through regulated substrate proteolysis. Proteolytic processing of the encoded protein results in the release of a small peptide, P-beta, which may transit to the nucleus. Mutations in this gene may be associated with Parkinson's disease.
Entrez Gene ID
55486
UniProt ID
Q9H300
Alternative Names
Presenilin Associated Rhomboid Like; EC 3.4.21.105; PSARL; Presenilins-Associated Rhomboid-Like Protein, Mitochondrial; Mitochondrial Intramembrane-Cleaving Protease PARL; Mitochondrial Intramembrane Cleaving Protease PARL; Presenilin Associated, Rhomboid-Like;
Function
Required for the control of apoptosis during postnatal growth. Essential for proteolytic processing of an antiapoptotic form of OPA1 which prevents the release of mitochondrial cytochrome c in response to intrinsic apoptotic signals (By similarity).
Required for the maturation of PINK1 into its 52kDa mature form after its cleavage by mitochondrial-processing peptidase (MPP) (PubMed:22354088).
Promotes changes in mitochondria morphology regulated by phosphorylation of P-beta domain (PubMed:14732705, PubMed:17116872).
Biological Process
Membrane protein proteolysisManual Assertion Based On ExperimentIDA:UniProtKB
Mitochondrial fusionIEA:Ensembl
Negative regulation of intrinsic apoptotic signaling pathwayIEA:Ensembl
Negative regulation of release of cytochrome c from mitochondriaIEA:Ensembl
ProteolysisManual Assertion Based On ExperimentIMP:ParkinsonsUK-UCL
Regulation of autophagy of mitochondrionIEA:Ensembl
Regulation of mitochondrion organizationManual Assertion Based On ExperimentIMP:ParkinsonsUK-UCL
Regulation of protein targeting to mitochondrionManual Assertion Based On ExperimentIGI:ParkinsonsUK-UCL
Regulation of proteolysisManual Assertion Based On ExperimentIGI:ParkinsonsUK-UCL
Regulation of reactive oxygen species metabolic processManual Assertion Based On ExperimentIMP:ParkinsonsUK-UCL
Signal peptide processingManual Assertion Based On ExperimentIBA:GO_Central
Cellular Location
Mitochondrion inner membrane
P-beta
Nucleus
Translocated into the nucleus by an unknown mechanism (PubMed:17116872).
Topology
Mitochondrial matrix: 53-101
Helical: 102-121
Mitochondrial intermembrane: 122-167
Helical: 168-187
Mitochondrial matrix: 188-207
Helical: 208-230
Mitochondrial intermembrane: 231-244
Helical: 245-262
Mitochondrial matrix: 263-272
Helical: 273-289
Mitochondrial intermembrane: 290-295
Helical: 296-318
Mitochondrial matrix: 319-332
Helical: 333-354
Mitochondrial intermembrane: 355-379
PTM
P-beta is proteolytically processed (beta-cleavage) in a PARL-dependent manner. The cleavage is inhibited when residues Ser-65, Thr-69 and Ser-70 are all phosphorylated.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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