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Mouse Anti-PARP14 Recombinant Antibody (C-1) (CBMAB-P0813-YC)

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Datasheet
SDS
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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal
Mouse
Clone
C-1
Application
WB, IP, IF, ELISA
Specificity
Human
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.
More Infomation

Target

Full Name
Poly(ADP-Ribose) Polymerase Family Member 14
Introduction
PARP14 is a member of the poly(ADP-ribose) polymerase (PARP) protein family. The encoded anti-apoptotic protein may regulate aerobic glycolysis and promote survival of cancer cells. Increased expression of this gene has been reported in a variety of tumor types.
Entrez Gene ID
54625
UniProt ID
Q460N5
Alternative Names
Poly(ADP-Ribose) Polymerase Family Member 14; ADP-Ribosyltransferase Diphtheria Toxin-Like 8; B Aggressive Lymphoma Protein 2; EC 2.4.2.30; PARP-14; ARTD8; BAL2;
Function
ADP-ribosyltransferase that mediates mono-ADP-ribosylation of glutamate residues on target proteins (PubMed:16061477, PubMed:27796300, PubMed:18851833, PubMed:25043379).
In contrast to PARP1 and PARP2, it is not able to mediate poly-ADP-ribosylation (PubMed:25043379).
Has been shown to catalyze the mono-ADP-ribosylation of STAT1 at 'Glu-657' and 'Glu-705', thus decreasing STAT1 phosphorylation which negatively regulates pro-inflammatory cytokine production in macrophages in response to IFNG stimulation (PubMed:27796300).
However, the role of ADP-ribosylation in the prevention of STAT1 phosphorylation has been called into question and it has been suggested that the inhibition of phosphorylation may be the result of sumoylation of STAT1 'Lys-703' (PubMed:29858569).
Mono-ADP-ribosylates STAT6; enhancing STAT6-dependent transcription (PubMed:27796300).
In macrophages, positively regulates MRC1 expression in response to IL4 stimulation by promoting STAT6 phosphorylation (PubMed:27796300).
Mono-ADP-ribosylates PARP9 (PubMed:27796300).
Biological Process
Innate immune responseIEA:UniProtKB-KW
Negative regulation of gene expressionManual Assertion Based On ExperimentIMP:UniProtKB
Negative regulation of interferon-gamma-mediated signaling pathwayManual Assertion Based On ExperimentIMP:UniProtKB
Negative regulation of tyrosine phosphorylation of STAT proteinManual Assertion Based On ExperimentIMP:UniProtKB
Positive regulation of interleukin-4-mediated signaling pathwayManual Assertion Based On ExperimentIMP:UniProtKB
Positive regulation of tyrosine phosphorylation of STAT proteinManual Assertion Based On ExperimentIMP:UniProtKB
Protein ADP-ribosylationManual Assertion Based On ExperimentIDA:UniProtKB
Protein mono-ADP-ribosylationManual Assertion Based On ExperimentIDA:UniProtKB
Protein poly-ADP-ribosylationManual Assertion Based On ExperimentIMP:UniProtKB
Cellular Location
Nucleus
Cytoplasm
In steady state splenocytes the protein is mostly nuclear (By similarity).
A minor proportion is detected in the cytoplasm (By similarity).
In macrophages, mainly localizes to the cytoplasm (PubMed:27796300).
PTM
Auto-ADP-ribosylated.

Torretta, A., Chatzicharalampous, C., Ebenwaldner, C., & Schüler, H. (2023). PARP14 is a writer, reader, and eraser of mono-ADP-ribosylation. Journal of Biological Chemistry, 299(9).

Dhoonmoon, A., & Nicolae, C. M. (2023). Mono-ADP-ribosylation by PARP10 and PARP14 in genome stability. NAR cancer, 5(1), zcad009.

Đukić, N., Strømland, Ø., Elsborg, J. D., Munnur, D., Zhu, K., Schuller, M., ... & Ahel, I. (2023). PARP14 is a PARP with both ADP-ribosyl transferase and hydrolase activities. Science Advances, 9(37), eadi2687.

Parthasarathy, S., & Fehr, A. R. (2022). PARP14: a key ADP-ribosylating protein in host–virus interactions?. PLoS pathogens, 18(6), e1010535.

Tang, Y., Liu, J., Wang, Y., Yang, L., Han, B., Zhang, Y., ... & Yao, H. (2021). PARP14 inhibits microglial activation via LPAR5 to promote post-stroke functional recovery. Autophagy, 17(10), 2905-2922.

Schenkel, L. B., Molina, J. R., Swinger, K. K., Abo, R., Blackwell, D. J., Lu, A. Z., ... & Kuntz, K. W. (2021). A potent and selective PARP14 inhibitor decreases protumor macrophage gene expression and elicits inflammatory responses in tumor explants. Cell chemical biology, 28(8), 1158-1168.

O’Connor, M. J., Thakar, T., Nicolae, C. M., & Moldovan, G. L. (2021). PARP14 regulates cyclin D1 expression to promote cell-cycle progression. Oncogene, 40(30), 4872-4883.

Tauber, A. L., Levonis, S. M., & Schweiker, S. S. (2020). Recent developments in PARP14 research. Future Medicinal Chemistry, 12(18), 1657-1667.

Schenkel, L., Molina, J., Swinger, K., Abo, R., Blackwell, D., Cheung, A., ... & Kuntz, K. (2020). A potent and selective PARP14 inhibitor decreases pro-tumor macrophage function and elicits inflammatory responses in tumor explants. Cancer Research, 80(16_Supplement), 1038-1038.

Qin, W., Wu, H. J., Cao, L. Q., Li, H. J., He, C. X., Zhao, D., ... & Cao, H. L. (2019). Research progress on PARP14 as a drug target. Frontiers in pharmacology, 10, 172.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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For research use only. Not intended for any clinical use.

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