Mouse Anti-PCGF6 Antibody (V2-5046) (CBMAB-0794-YC)

Name: Mouse Anti-PCGF6 Antibody (V2-5046)
SKU: CBMAB-0794-YC
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

V2-5046

Application

Immunogen

Recombinant peptide

Specificity

Human

Antibody Isotype

IgG2b

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Supernatant

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

PCGF6

Introduction

PcG proteins are known to form protein complexes and function as transcription repressors. PCGF6 (polycomb group ring finger 6) contains a RING finger motif, which is most closely related to those of polycomb group (PcG) proteins RNF110/MEL-18 and BMI1. PCGF6 has been shown to interact with some PcG proteins and act as a transcription repressor and is found to be regulated by cell cycle dependent phosphorylation.

Entrez Gene ID

84108

UniProt ID

Q9BYE7

Alternative Names

MBLR; RNF134

Function

Transcriptional repressor (PubMed:12167161).
May modulate the levels of histone H3K4Me3 by activating KDM5D histone demethylase (PubMed:17320162).
Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility (PubMed:12167161).
Within the PRC1-like complex, regulates RNF2 ubiquitin ligase activity (PubMed:26151332).

Biological Process

Histone H2A-K119 monoubiquitinationManual Assertion Based On ExperimentIMP:UniProtKB
Negative regulation of transcription by RNA polymerase IIIEA:Ensembl
Negative regulation of transcription, DNA-templatedManual Assertion Based On ExperimentIDA:UniProtKB
Regulation of transcription by RNA polymerase IIManual Assertion Based On ExperimentIBA:GO_Central

Cellular Location

Nucleus

PTM

Phosphorylated during mitosis. Phosphorylated on Ser-30 by CDK7 in vitro.

Del Vecchio, A., Mulé, P., Fernández-Pérez, D., Amato, S., Lattanzi, G., Zanotti, M., ... & Pasini, D. (2024). PCGF6 controls murine Tuft cell differentiation via H3K9me2 modification independently of Polycomb repression. Developmental Cell, 59(3), 368-383.

Zhu, M., Zhang, R. N., Zhang, H., Qu, C. B., Zhang, X. C., Ren, L. X., ... & Gu, J. F. (2023). PCGF6/MAX/KDM5D facilitates MAZ/CDK4 axis expression and pRCC progression by hypomethylation of the DNA promoter. Epigenetics & Chromatin, 16(1), 9.

Lan, X., Ding, S., Zhang, T., Yi, Y., Li, C., Jin, W., ... & Jiang, W. (2022). PCGF6 controls neuroectoderm specification of human pluripotent stem cells by activating SOX2 expression. Nature Communications, 13(1), 4601.

Liu, M., Zhu, Y., Xing, F., Liu, S., Xia, Y., Jiang, Q., & Qin, J. (2020). The polycomb group protein PCGF6 mediates germline gene silencing by recruiting histone-modifying proteins to target gene promoters. Journal of Biological Chemistry, 295(28), 9712-9724.

Huang, X., Wei, C., Li, F., Jia, L., Zeng, P., Li, J., ... & Ding, J. (2019). PCGF6 regulates stem cell pluripotency as a transcription activator via super-enhancer dependent chromatin interactions. Protein & cell, 10(10), 709-725.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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