Mouse Anti-PDE8A Recombinant Antibody (1H6) (CBMAB-P1230-YC)

Name: Mouse Anti-PDE8A Recombinant Antibody (1H6)
SKU: CBMAB-P1230-YC
Rating: 5 (1 reviews)

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Datasheet

SDS

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Basic Information

Host Animal

Mouse

Clone

1H6

Application

ELISA, WB

Immunogen

PDE8A (NP_002596.1, 32-121 aa) partial recombinant protein with GST tag. Immunogen sequence: RLPQGQKTAA LPRTRGAGLL ESELRDGSGK KVAVADVQFG PMRFHQDQLQ VLLVFTKEDN QCNGFCRACE KAGFKCTVTK EAQAVLACFL

Specificity

Human

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.

Epitope

aa 32-121

More Infomation

Target

Full Name

phosphodiesterase 8A

Introduction

PDE8A belongs to the cyclic nucleotide phosphodiesterase (PDE) family, and PDE8 subfamily. This PDE hydrolyzes the second messenger, cAMP, which is a regulator and mediator of a number of cellular responses to extracellular signals. Thus, by regulating the cellular concentration of cAMP, this protein plays a key role in many important physiological processes.

Entrez Gene ID

5151

UniProt ID

O60658

Alternative Names

Phosphodiesterase 8A; High Affinity CAMP-Specific And IBMX-Insensitive 3,5-Cyclic Phosphodiesterase 8A; CAMP-Specific Cyclic Nucleotide Phosphodiesterase 8A; EC 3.1.4.17; EC 3.1.4.53; HsT19550;

Function

Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes (PubMed:18983167).
May be involved in maintaining basal levels of the cyclic nucleotide and/or in the cAMP regulation of germ cell development (PubMed:18983167).
Binding to RAF1 reduces RAF1 'Ser-259' inhibitory-phosphorylation and stimulates RAF1-dependent EGF-activated ERK-signaling (PubMed:23509299).
Protects against cell death induced by hydrogen peroxide and staurosporine (PubMed:23509299).

Biological Process

cAMP catabolic processIEA:UniProtKB-UniPathway
Cellular response to epidermal growth factor stimulusManual Assertion Based On ExperimentIMP:UniProtKB
Negative regulation of cell deathManual Assertion Based On ExperimentIMP:UniProtKB
Negative regulation of hydrogen peroxide-induced cell deathManual Assertion Based On ExperimentIMP:UniProtKB
Positive regulation of ERK1 and ERK2 cascadeManual Assertion Based On ExperimentIMP:UniProtKB
Positive regulation of protein phosphorylationManual Assertion Based On ExperimentIMP:UniProtKB
Regulation of transcription, DNA-templatedIEA:InterPro
Signal transductionManual Assertion Based On ExperimentIBA:GO_Central

Cellular Location

cytosol
extracellular exosome

PTM

Phosphorylated at Ser-359 by PKA under elevated cAMP conditions, this enhances catalytic activity.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Related Products

Mouse Anti-PDE8A Recombinant Antibody (EM-52) (CAT#: CBMAB-P1231-YC)

Isotype control

For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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