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Mouse Anti-PDK4 Recombinant Antibody (1A9) (CBMAB-P1324-YC)

Provided herein is a Mouse monoclonal antibody against Human Pyruvate Dehydrogenase Kinase 4. The antibody can be used for immunoassay techniques, such as ELISA.
See all PDK4 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
1A9
Antibody Isotype
IgG2b, κ
Application
ELISA

Basic Information

Immunogen
PDK4 (AAH40239.1, 1-411 aa) full length recombinant protein with GST tag. Immunogen sequence: MKAARFVLRS AGSLNGAGLV PREVEHFSRY SPSPLSMKQL LDFGSENACE RTSFAFLRQE LPVRLANILK EIDILPTQLV NTSSVQLVKS WYIQSLMDLV EFHEKSPDDQ KALSDFVDTL IKVRNRHHNV VPTMAQGIIE YKDACTVDPV TNQNLQYFLD RFYMNRISTR MLMNQHILIF SDSQTGNPSH IGSIDPNCDV VAVVQDAFEC SRMLCDQYYL SSPELKLTQV NGKFPDQPIH IVYVPSHLHH MLFELFKNAM RATVEHQENQ PSLTPIEVIV VLGKEDLTIK ISDRGGGVPL RIIDRLFSYT YSTAPTPVMD NSRNAPLAGF GYGLPISRLY AKYFQGDLNL YSLSGYGTDA IIYLKALSSE SIEKLPVFNK SAFKHYQMSS EADDWCIPSR EPKNLAKEVA M
Specificity
Human
Antibody Isotype
IgG2b, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
pyruvate dehydrogenase kinase, isozyme 4
Introduction
PDK4 is a member of the PDK/BCKDK protein kinase family and encodes a mitochondrial protein with a histidine kinase domain. This protein is located in the matrix of the mitrochondria and inhibits the pyruvate dehydrogenase complex by phosphorylating one of its subunits, thereby contributing to the regulation of glucose metabolism. Expression of this gene is regulated by glucocorticoids, retinoic acid and insulin.
Entrez Gene ID
5166
UniProt ID
Q16654
Alternative Names
Pyruvate Dehydrogenase Kinase 4; EC 2.7.11.2; Pyruvate Dehydrogenase Kinase, Isoenzyme 4; Pyruvate Dehydrogenase Kinase, Isozyme 4; Pyruvate Dehydrogenase Kinase Isoform 4; EC 2.7.11; PDHK4;
Function
Kinase that plays a key role in regulation of glucose and fatty acid metabolism and homeostasis via phosphorylation of the pyruvate dehydrogenase subunits PDHA1 and PDHA2. This inhibits pyruvate dehydrogenase activity, and thereby regulates metabolite flux through the tricarboxylic acid cycle, down-regulates aerobic respiration and inhibits the formation of acetyl-coenzyme A from pyruvate. Inhibition of pyruvate dehydrogenase decreases glucose utilization and increases fat metabolism in response to prolonged fasting and starvation. Plays an important role in maintaining normal blood glucose levels under starvation, and is involved in the insulin signaling cascade. Via its regulation of pyruvate dehydrogenase activity, plays an important role in maintaining normal blood pH and in preventing the accumulation of ketone bodies under starvation. In the fed state, mediates cellular responses to glucose levels and to a high-fat diet. Regulates both fatty acid oxidation and de novo fatty acid biosynthesis. Plays a role in the generation of reactive oxygen species. Protects detached epithelial cells against anoikis. Plays a role in cell proliferation via its role in regulating carbohydrate and fatty acid metabolism.
Biological Process
Cellular response to fatty acidManual Assertion Based On ExperimentIMP:UniProtKB
Cellular response to starvationManual Assertion Based On ExperimentIDA:UniProtKB
Glucose homeostasisISS:UniProtKB
Glucose metabolic processIEA:UniProtKB-KW
Insulin receptor signaling pathwayManual Assertion Based On ExperimentIMP:UniProtKB
Negative regulation of anoikisManual Assertion Based On ExperimentIMP:UniProtKB
Protein phosphorylationManual Assertion Based On ExperimentIBA:GO_Central
Reactive oxygen species metabolic processManual Assertion Based On ExperimentIMP:UniProtKB
Regulation of acetyl-CoA biosynthetic process from pyruvateManual Assertion Based On ExperimentIMP:UniProtKB
Regulation of bone resorptionIEA:Ensembl
Regulation of cellular ketone metabolic processISS:UniProtKB
Regulation of fatty acid biosynthetic processManual Assertion Based On ExperimentIMP:UniProtKB
Regulation of fatty acid oxidationISS:UniProtKB
Regulation of glucose metabolic processManual Assertion Based On ExperimentIMP:UniProtKB
Regulation of pHISS:UniProtKB
Response to starvationISS:UniProtKB
Cellular Location
Mitochondrion matrix
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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