Mouse Anti-PFN1 Recombinant Antibody (2D2) (CBMAB-P1524-YC)

Name: Mouse Anti-PFN1 Recombinant Antibody (2D2)
SKU: CBMAB-P1524-YC
Rating: 5 (1 reviews)

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Datasheet

SDS

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Basic Information

Host Animal

Mouse

Clone

2D2

Application

FC, ICC, IF, IHC, IHC-P, WB

Immunogen

Full length human recombinant protein of human PFN1 (NP_005013) produced in HEK293T cell

Specificity

Human, Dog, Monkey, Rat

Antibody Isotype

IgG1

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

PBS, pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Profilin 1

Introduction

PFN1 is a member of the profilin family of small actin-binding proteins. The encoded protein plays an important role in actin dynamics by regulating actin polymerization in response to extracellular signals. Deletion of this gene is associated with Miller-Dieker syndrome, and the encoded protein may also play a role in Huntington disease. Multiple pseudogenes of this gene are located on chromosome 1.

Entrez Gene ID

Human	5216
Dog	607397
Rat	64303
Monkey	710753

UniProt ID

Human	P07737
Dog	F1Q3Y0
Rat	P62963
Monkey	F7H2A5

Alternative Names

Profilin 1; Epididymis Tissue Protein Li 184a; Profilin I; Profilin-1; ALS18;

Function

Binds to actin and affects the structure of the cytoskeleton. At high concentrations, profilin prevents the polymerization of actin, whereas it enhances it at low concentrations. By binding to PIP2, it inhibits the formation of IP3 and DG. Inhibits androgen receptor (AR) and HTT aggregation and binding of G-actin is essential for its inhibition of AR.

Biological Process

Actin cytoskeleton organizationIEA:InterPro
Modification of postsynaptic actin cytoskeletonIEA:Ensembl
Negative regulation of actin filament bundle assemblyManual Assertion Based On ExperimentIMP:UniProtKB
Negative regulation of actin filament polymerizationManual Assertion Based On ExperimentIDA:UniProtKB
Negative regulation of stress fiber assemblyManual Assertion Based On ExperimentIMP:UniProtKB
Neural tube closureIEA:Ensembl
Positive regulation of actin filament bundle assemblyManual Assertion Based On ExperimentIBA:GO_Central
Positive regulation of actin filament polymerizationManual Assertion Based On ExperimentIGI:UniProtKB
Positive regulation of ATP-dependent activityManual Assertion Based On ExperimentIDA:UniProtKB
Positive regulation of epithelial cell migrationManual Assertion Based On ExperimentIMP:UniProtKB
Positive regulation of ruffle assemblyManual Assertion Based On ExperimentIMP:UniProtKB
Protein stabilizationManual Assertion Based On ExperimentIDA:UniProtKB
Regulation of actin filament polymerizationManual Assertion Based On ExperimentIBA:GO_Central
Regulation of transcription by RNA polymerase IIIEA:Ensembl
Synapse maturationIEA:Ensembl

Cellular Location

Cytoplasm, cytoskeleton

Involvement in disease

Amyotrophic lateral sclerosis 18 (ALS18):
A neurodegenerative disorder affecting upper motor neurons in the brain and lower motor neurons in the brain stem and spinal cord, resulting in fatal paralysis. Sensory abnormalities are absent. The pathologic hallmarks of the disease include pallor of the corticospinal tract due to loss of motor neurons, presence of ubiquitin-positive inclusions within surviving motor neurons, and deposition of pathologic aggregates. The etiology of amyotrophic lateral sclerosis is likely to be multifactorial, involving both genetic and environmental factors. The disease is inherited in 5-10% of the cases.

PTM

Phosphorylation at Ser-138 reduces its affinity for G-actin and blocks its interaction with HTT, reducing its ability to inhibit androgen receptor (AR) and HTT aggregation.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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