Mouse Anti-PGAM5 Recombinant Antibody (CL0624) (CBMAB-P1551-YC)

Name: Mouse Anti-PGAM5 Recombinant Antibody (CL0624)
SKU: CBMAB-P1551-YC
Rating: 5 (1 reviews)

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Datasheet

SDS

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Basic Information

Host Animal

Mouse

Clone

CL0624

Application

IHC, WB

Immunogen

Recombinant protein corresponding to Human PGAM5

Specificity

Human

Antibody Isotype

IgG1

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

PBS with 40% glycerol and 0.02% sodium azide, pH 7.2

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

PGAM Family Member 5, Mitochondrial Serine/Threonine Protein Phosphatase

Introduction

PGAM5 has ubiquitous expression in esophagus (RPKM 6.8), testis (RPKM 6.7) and 25 other tissues.

Entrez Gene ID

192111

UniProt ID

Q96HS1

Alternative Names

PGAM Family Member 5, Mitochondrial Serine/Threonine Protein Phosphatase; Phosphoglycerate Mutase Family Member 5; PGAM Family Member 5, Serine/Threonine Protein Phosphatase, Mitochondrial; Bcl-XL-Binding Protein V68; Serine/Threonine-Protein Phosphatase PGAM5, Mitochondrial; EC 3.1.3.16; BXLBV68;

Function

Displays phosphatase activity for serine/threonine residues, and, dephosphorylates and activates MAP3K5 kinase. Has apparently no phosphoglycerate mutase activity. May be regulator of mitochondrial dynamics. Substrate for a KEAP1-dependent ubiquitin ligase complex. Contributes to the repression of NFE2L2-dependent gene expression. Acts as a central mediator for programmed necrosis induced by TNF, by reactive oxygen species and by calcium ionophore.

Biological Process

MacroautophagyTAS:Reactome
Necroptotic processManual Assertion Based On ExperimentIMP:UniProtKB
Negative regulation of cold-induced thermogenesisBy SimilarityISS:YuBioLab
Peptidyl-threonine dephosphorylationManual Assertion Based On ExperimentIBA:GO_Central
Positive regulation of mitochondrial fissionManual Assertion Based On ExperimentIBA:GO_Central

Cellular Location

Mitochondrion outer membrane
Isoform 2 overexpression results in the formation of disconnected punctuate mitochondria distributed throughout the cytoplasm. Isoform 1 overexpression results in the clustering of mitochondria around the nucleus.

Topology

Helical: 7-29

PTM

Both isoform 1 and isoform 2 are phosphorylated by the RIPK1/RIPK3 complex under necrotic conditions. This phosphorylation increases PGAM5 phosphatase activity.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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