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Mouse Anti-PHF19 Antibody (2B8) (CBMAB-0804-YC)

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Basic Information

Host Animal
Mouse
Clone
2B8
Application
IP, WB
Immunogen
Recombinant protein
Specificity
Human
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Supernatant
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
More Infomation

Target

Full Name
PHF19
Introduction
PHF19 (PHD finger protein 19) is a protein that has methylated histone binding activity. Diseases associated with PHF19 include cataract 7 and leukoencephalopathy with vanishing white matter. Among its related pathways are gene expression and chromatin regulation / acetylation. An important paralog of this gene is MTF2.
Entrez Gene ID
26147
UniProt ID
Q5T6S3
Alternative Names
DKFZP727G051; MTF2L1; PCL3; TDRD19B
Function
Polycomb group (PcG) protein that specifically binds histone H3 trimethylated at 'Lys-36' (H3K36me3) and recruits the PRC2 complex, thus enhancing PRC2 H3K27me3 methylation activity (PubMed:15563832, PubMed:18691976, PubMed:23160351, PubMed:23228662, PubMed:23273982, PubMed:29499137, PubMed:23104054, PubMed:31959557).
Probably involved in the transition from an active state to a repressed state in embryonic stem cells: acts by binding to H3K36me3, a mark for transcriptional activation, and recruiting H3K36me3 histone demethylases RIOX1 or KDM2B, leading to demethylation of H3K36 and recruitment of the PRC2 complex that mediates H3K27me3 methylation, followed by de novo silencing (PubMed:23160351).
Recruits the PRC2 complex to CpG islands and contributes to embryonic stem cell self-renewal. Also binds histone H3 dimethylated at 'Lys-36' (H3K36me2) (PubMed:23104054).
Isoform 1 and isoform 2 inhibit transcription from an HSV-tk promoter (PubMed:15563832).
Biological Process
Chromatin organizationManual Assertion Based On ExperimentIBA:GO_Central
Negative regulation of transcription by RNA polymerase IIIEA:Ensembl
Positive regulation of histone H3-K27 methylationManual Assertion Based On ExperimentIMP:UniProtKB
Regulation of transcription, DNA-templatedManual Assertion Based On ExperimentIBA:GO_Central
Stem cell differentiationIEA:Ensembl
Stem cell population maintenanceIEA:Ensembl
Cellular Location
Nucleus
Localizes to chromatin as part of the PRC2 complex.
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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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