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Mouse Anti-PIAS3 Recombinant Antibody (4F12) (CBMAB-P1742-YC)

Provided herein is a Mouse monoclonal antibody against Human Protein Inhibitor Of Activated STAT 3. The antibody can be used for immunoassay techniques, such as WB, ELISA.
See all PIAS3 antibodies
Published Data
Fig.1 The expression of ATBF1, PIAS3, p-STAT3 and STAT3 protein was evaluated by Western blot.
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Fig.2 After 24 h of electrical stimulation and 72 h of transfection with pKXUa1-HA-ATBF1, the expression of ATBF1, PIAS3, p-STAT3, STAT3 and b-actin was measured with Western blot analysis.
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Fig.3 Co-transfection of ATBF1 siRNA and PIAS3 siRNA activates the STAT3 signaling in HL-1 cardiomyocytes with a synergistic effect.
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Fig.4 Co-IP was used to evaluate the binding of ATBF1, PIAS3, p-STAT3 and STAT3 with or without stimulation.
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Summary

Host Animal
Mouse
Specificity
Human
Clone
4F12
Antibody Isotype
IgG2a
Application
WB, ELISA

Basic Information

Immunogen
Recombinant protein corresponding to aa 453-551 of human PIAS3
Specificity
Human
Antibody Isotype
IgG2a
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Protein Inhibitor Of Activated STAT 3
Introduction
PIAS3 is a member of the PIAS [protein inhibitor of activated STAT (signal transducer and activator of transcription)] family of transcriptional modulators. The protein functions as a SUMO (small ubiquitin-like modifier)-E3 ligase which catalyzes the covalent attachment of a SUMO protein to specific target substrates. It directly binds to several transcription factors and either blocks or enhances their activity. Alternatively spliced transcript variants of this gene have been identified, but the full-length nature of some of these variants has not been determined.
Entrez Gene ID
10401
UniProt ID
Q9Y6X2
Alternative Names
Protein Inhibitor Of Activated STAT 3; Protein Inhibitor Of Activated STAT Protein 3; Zinc Finger, MIZ-Type Containing 5; E3 SUMO-Protein Ligase PIAS3; EC 6.3.2.-; ZMIZ5;
Function
Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway and the steroid hormone signaling pathway. Involved in regulating STAT3 signaling via inhibiting STAT3 DNA-binding and suppressing cell growth. Enhances the sumoylation of MTA1 and may participate in its paralog-selective sumoylation (PubMed:21965678, PubMed:9388184).
Sumoylates CCAR2 which promotes its interaction with SIRT1 (PubMed:25406032).
Diminishes the sumoylation of ZFHX3 by preventing the colocalization of ZFHX3 with SUMO1 in the nucleus (PubMed:24651376).
Biological Process
Negative regulation of gene expressionIEA:Ensembl
Negative regulation of osteoclast differentiationIEA:Ensembl
Negative regulation of protein sumoylationManual Assertion Based On ExperimentIDA:UniProtKB
Negative regulation of transcription by RNA polymerase IIIEA:Ensembl
Positive regulation of gene expressionIEA:Ensembl
Positive regulation of membrane potentialIEA:Ensembl
Positive regulation of protein sumoylationManual Assertion Based On ExperimentIDA:UniProtKB
Protein sumoylationISS:UniProtKB
Regulation of transcription by RNA polymerase IIManual Assertion Based On ExperimentIBA:GO_Central
Response to hormoneIEA:Ensembl
TNFSF11-mediated signaling pathwayIEA:Ensembl
Cellular Location
Cytoplasm
Nucleus
Nucleus speckle
Colocalizes with MITF in the nucleus. Colocalizes with GFI1 in nuclear dots. Colocalizes with SUMO1 in nuclear granules.
PTM
Sumoylated.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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