Rabbit Anti-PIAS3 Recombinant Antibody (D5F9) (CBMAB-P1743-YC)

Name: Rabbit Anti-PIAS3 Recombinant Antibody (D5F9)
SKU: CBMAB-P1743-YC
Rating: 5 (1 reviews)

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Datasheet

SDS

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Basic Information

Host Animal

Rabbit

Clone

D5F9

Application

WB, IP, IF

Specificity

Human, Monkey

Antibody Isotype

IgG

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Protein Inhibitor Of Activated STAT 3

Introduction

PIAS3 is a member of the PIAS [protein inhibitor of activated STAT (signal transducer and activator of transcription)] family of transcriptional modulators. The protein functions as a SUMO (small ubiquitin-like modifier)-E3 ligase which catalyzes the covalent attachment of a SUMO protein to specific target substrates. It directly binds to several transcription factors and either blocks or enhances their activity. Alternatively spliced transcript variants of this gene have been identified, but the full-length nature of some of these variants has not been determined.

Entrez Gene ID

Human	10401
Monkey	706775

UniProt ID

Human	Q9Y6X2
Monkey	H9FNP4

Alternative Names

Protein Inhibitor Of Activated STAT 3; Protein Inhibitor Of Activated STAT Protein 3; Zinc Finger, MIZ-Type Containing 5; E3 SUMO-Protein Ligase PIAS3; EC 6.3.2.-; ZMIZ5;

Function

Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway and the steroid hormone signaling pathway. Involved in regulating STAT3 signaling via inhibiting STAT3 DNA-binding and suppressing cell growth. Enhances the sumoylation of MTA1 and may participate in its paralog-selective sumoylation (PubMed:21965678, PubMed:9388184).
Sumoylates CCAR2 which promotes its interaction with SIRT1 (PubMed:25406032).
Diminishes the sumoylation of ZFHX3 by preventing the colocalization of ZFHX3 with SUMO1 in the nucleus (PubMed:24651376).

Biological Process

Negative regulation of gene expressionIEA:Ensembl
Negative regulation of osteoclast differentiationIEA:Ensembl
Negative regulation of protein sumoylationManual Assertion Based On ExperimentIDA:UniProtKB
Negative regulation of transcription by RNA polymerase IIIEA:Ensembl
Positive regulation of gene expressionIEA:Ensembl
Positive regulation of membrane potentialIEA:Ensembl
Positive regulation of protein sumoylationManual Assertion Based On ExperimentIDA:UniProtKB
Protein sumoylationISS:UniProtKB
Regulation of transcription by RNA polymerase IIManual Assertion Based On ExperimentIBA:GO_Central
Response to hormoneIEA:Ensembl
TNFSF11-mediated signaling pathwayIEA:Ensembl

Cellular Location

Cytoplasm
Nucleus
Nucleus speckle
Colocalizes with MITF in the nucleus. Colocalizes with GFI1 in nuclear dots. Colocalizes with SUMO1 in nuclear granules.

PTM

Sumoylated.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Related Products

Mouse Anti-PIAS3 Recombinant Antibody (4F12) (CAT#: CBMAB-P1742-YC)

Mouse Anti-PIAS3 Recombinant Antibody (PIA3) (CAT#: CBMAB-P1744-YC)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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