Mouse Anti-PIM2 Recombinant Antibody (5B5) (CBMAB-P1851-YC)

Name: Mouse Anti-PIM2 Recombinant Antibody (5B5)
SKU: CBMAB-P1851-YC
Rating: 5 (1 reviews)

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Datasheet

SDS

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Basic Information

Host Animal

Mouse

Clone

5B5

Application

FC, ICC, IF, IHC, IHC-P, IP, WB

Immunogen

Full length human recombinant protein of human PIM2 (NP_006866) produced in HEK293T cell

Specificity

Human

Antibody Isotype

IgG2a

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

PBS, pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Pim-2 Proto-Oncogene, Serine/Threonine Kinase

Introduction

PIM2 is a protooncogene that acts as a serine/threonine protein kinase. Studies determined the encoded protein functions to prevent apoptosis and to promote cell survival.

Entrez Gene ID

11040

UniProt ID

Q9P1W9

Alternative Names

Pim-2 Proto-Oncogene, Serine/Threonine Kinase; Pim-2 Oncogene; EC 2.7.11.1; Pim-2h; Proto-Oncogene Pim-2 (Serine Threonine Kinase); Serine/Threonine-Protein Kinase Pim-2;

Function

Proto-oncogene with serine/threonine kinase activity involved in cell survival and cell proliferation. Exerts its oncogenic activity through: the regulation of MYC transcriptional activity, the regulation of cell cycle progression, the regulation of cap-dependent protein translation and through survival signaling by phosphorylation of a pro-apoptotic protein, BAD. Phosphorylation of MYC leads to an increase of MYC protein stability and thereby an increase transcriptional activity. The stabilization of MYC exerted by PIM2 might explain partly the strong synergism between these 2 oncogenes in tumorigenesis. Regulates cap-dependent protein translation in a mammalian target of rapamycin complex 1 (mTORC1)-independent manner and in parallel to the PI3K-Akt pathway. Mediates survival signaling through phosphorylation of BAD, which induces release of the anti-apoptotic protein Bcl-X(L)/BCL2L1. Promotes cell survival in response to a variety of proliferative signals via positive regulation of the I-kappa-B kinase/NF-kappa-B cascade; this process requires phosphorylation of MAP3K8/COT. Promotes growth factor-independent proliferation by phosphorylation of cell cycle factors such as CDKN1A and CDKN1B. Involved in the positive regulation of chondrocyte survival and autophagy in the epiphyseal growth plate.

Biological Process

Apoptotic processIEA:UniProtKB-KW
G1/S transition of mitotic cell cycleManual Assertion Based On ExperimentIDA:UniProtKB
Negative regulation of apoptotic processManual Assertion Based On ExperimentIDA:UniProtKB
Negative regulation of cell population proliferationManual Assertion Based On ExperimentIDA:UniProtKB
Positive regulation of autophagyISS:UniProtKB
Positive regulation of I-kappaB kinase/NF-kappaB signalingISS:UniProtKB
Positive regulation of transcription, DNA-templatedISS:UniProtKB
Protein autophosphorylationManual Assertion Based On ExperimentIBA:GO_Central
Protein phosphorylationManual Assertion Based On ExperimentIDA:UniProtKB
Protein stabilizationManual Assertion Based On ExperimentIMP:UniProtKB
Regulation of mitotic cell cycleManual Assertion Based On ExperimentIBA:GO_Central
Response to virusManual Assertion Based On ExperimentIEP:UniProtKB

Cellular Location

Cytoplasm

PTM

Autophosphorylated.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Mouse Anti-PIM2 Recombinant Antibody (5D5) (CAT#: CBMAB-P1852-YC)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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