Rabbit Anti-PIN4 Recombinant Antibody (
14A7) (V2LY-0725-LY1468)

Name: Rabbit Anti-PIN4 Recombinant Antibody (14A7)
SKU: V2LY-0725-LY1468
Rating: 5 (1 reviews)

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Tested Data

Rabbit Anti-PIN4 Recombinant Antibody (14A7) (V2LY-0725-LY1468) in WB

Western Blot
Positive WB detected in: U251 whole cell lysate, MCF-7 whole cell lysate, HEK293 whole cell lysate, Hela whole cell lysate, Jurkat whole cell lysate, PC3 whole cell lysate
All lanes: PIN4 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 14, 17, 15 kDa
Observed band size: 12-17 kDa

Rabbit Anti-PIN4 Recombinant Antibody (14A7) (V2LY-0725-LY1468) in IHC

IHC image of V2LY-0725-LY1468 diluted at 1:100 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.

Rabbit Anti-PIN4 Recombinant Antibody (14A7) (V2LY-0725-LY1468) in IF

Immunofluorescence staining of MCF-7 cell with V2LY-0725-LY991 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 598-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Datasheet

SDS

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Basic Information

Host Animal

Rabbit

Clone

14A7

Application

ELISA, WB, IHC, IF

Immunogen

A synthesized peptide derived from human PIN4.

Host Species

Rabbit

Specificity

Human

Antibody Isotype

IgG

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:500-1:2,000
IHC	1:50-1:200
IF	1:50-1:200

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, glycerol

Preservative

Sodium azide

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Peptidylprolyl Cis/Trans Isomerase, NIMA-Interacting 4

Entrez Gene ID

5303

UniProt ID

Q9Y237

Function

Isoform 1 is involved as a ribosomal RNA processing factor in ribosome biogenesis. Binds to tightly bent AT-rich stretches of double-stranded DNA.
Isoform 2 binds to double-stranded DNA.

Biological Process

rRNA processingIMP:UniProtKB

Cellular Location

Isoform 1
Nucleus, nucleolus
Cytoplasm, cytoskeleton, spindle
Cytoplasm
Colocalizes in the nucleolus during interphase and on the spindle apparatus during mitosis with NPM1.
Isoform 2
Mitochondrion
Mitochondrion matrix
Imported in a time- and membrane potential-dependent manner to the mitochondrial matrix, but without concomitant processing of the protein. Directed to mitochondria by a novel N-terminal domain that functions as non-cleavable mitochondrial targeting peptide.

PTM

Phosphorylated. Isoform 1 phosphorylation occurs both in the nucleus and the cytoplasm. Isoform 1 phosphorylation at Ser-19 does not affect its PPIase activity but is required for nuclear localization, and the dephosphorylation is a prerequisite for the binding to DNA. The unphosphorylated isoform 1 associates with the pre-rRNP complexes in the nucleus.
Isoform 2 is sumoylated with SUMO2 and SUMO3.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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