Mouse Anti-PISD Recombinant Antibody (B-3) (CBMAB-P1891-YC)

Name: Mouse Anti-PISD Recombinant Antibody (B-3)
SKU: CBMAB-P1891-YC
Rating: 5 (1 reviews)

See all PISD antibodies

Compare Online Inquiry

Datasheet

SDS

Request for COA

Datasheet Target Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

B-3

Application

WB, IP, IF, ELISA

Specificity

Human

Antibody Isotype

IgG

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Phosphatidylserine decarboxylase

Introduction

PISD catalyzes the conversion of phosphatidylserine to phosphatidylethanolamine in the inner mitochondrial membrane. The encoded protein is active in phospholipid metabolism and interorganelle trafficking of phosphatidylserine.

Entrez Gene ID

23761

UniProt ID

Q9UG56

Alternative Names

DJ858B16; PSD; PSDC; PSSC; dJ858B16.2

Function

Catalyzes the formation of phosphatidylethanolamine (PtdEtn) from phosphatidylserine (PtdSer) (PubMed:30488656, PubMed:30858161).
Plays a central role in phospholipid metabolism and in the interorganelle trafficking of phosphatidylserine.

Biological Process

Mitochondrial protein catabolic processManual Assertion Based On ExperimentIMP:UniProtKB
Phosphatidylethanolamine biosynthetic processManual Assertion Based On ExperimentIMP:UniProtKB
Protein autoprocessingIEA:UniProtKB-UniRule
Regulation of mitochondrion organizationManual Assertion Based On ExperimentIMP:UniProtKB

Cellular Location

Phosphatidylserine decarboxylase beta chain
Mitochondrion inner membrane
Phosphatidylserine decarboxylase alpha chain
Mitochondrion inner membrane
Anchored to the mitochondrial inner membrane through its interaction with the integral membrane beta chain.

Involvement in disease

Liberfarb syndrome (LIBF):
An autosomal recessive multisystem disorder affecting the eye, ear, bone, and brain development. Clinical features include early-onset retinal degeneration, congenital cataracts, sensorineural hearing loss, microcephaly, intellectual disability, white matter changes, mild facial dysmorphism, and skeletal dysplasia with platyspondyly, scoliosis and short stature.

Topology

Mitochondrial matrix: 53-63
Helical: 64-82
Mitochondrial intermembrane: 83-409

PTM

Is synthesized initially as an inactive proenzyme. Formation of the active enzyme involves a self-maturation process in which the active site pyruvoyl group is generated from an internal serine residue via an autocatalytic post-translational modification. Two non-identical subunits are generated from the proenzyme in this reaction, and the pyruvate is formed at the N-terminus of the alpha chain, which is derived from the carboxyl end of the proenzyme. The autoendoproteolytic cleavage occurs by a canonical serine protease mechanism, in which the side chain hydroxyl group of the serine supplies its oxygen atom to form the C-terminus of the beta chain, while the remainder of the serine residue undergoes an oxidative deamination to produce ammonia and the pyruvoyl prosthetic group on the alpha chain. During this reaction, the Ser that is part of the protease active site of the proenzyme becomes the pyruvoyl prosthetic group, which constitutes an essential element of the active site of the mature decarboxylase.

Ask a question We look forward to hearing from you.

0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Have you used Mouse Anti-PISD Recombinant Antibody (B-3)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon

$30 eGift Card
Submit a review

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Related Products

Mouse Anti-PISD Recombinant Antibody (5D8) (CAT#: CBMAB-D2164-YC)

Mouse Anti-PISD Recombinant Antibody (3B11) (CAT#: CBMAB-P1889-YC)

Mouse Anti-PISD Recombinant Antibody (4G5) (CAT#: CBMAB-P1890-YC)

Mouse Anti-PISD Recombinant Antibody (2C1) (CAT#: CBMAB-D2162-YC)

Mouse Anti-PISD Recombinant Antibody (2H1) (CAT#: CBMAB-D2163-YC)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Request bulk or custom quote Second antibody and isotype control

Contact us

Tel: (USA)
(UK)
Fax:

Global Locations

Email:

Online Inquiry