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Mouse Anti-PIWIL4 (AA 304-434) Recombinant Antibody (10G9B11) (CBMAB-H4186-FY)


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Summary

Host Animal
Mouse
Specificity
Human
Clone
10G9B11
Antibody Isotype
IgG1
Application
ELISA, FC, IHC-P, WB

Basic Information

Specificity
Human
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS
Preservative
0.05% Sodium azide
Concentration
1 mg/mL
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Epitope
AA 304-434

Target

Full Name
Piwi like RNA-mediated gene silencing 4
Introduction
PIWIL4 belongs to the Argonaute family of proteins, which function in development and maintenance of germline stem cells.
Entrez Gene ID
143689
UniProt ID
Q7Z3Z4
Alternative Names
HIWI2; MIWI2
Function
Plays a central role during spermatogenesis by repressing transposable elements and preventing their mobilization, which is essential for the germline integrity (By similarity).
Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and governs the methylation and subsequent repression of transposons (By similarity).
Directly binds piRNAs, a class of 24 to 30 nucleotide RNAs that are generated by a Dicer-independent mechanism and are primarily derived from transposons and other repeated sequence elements (By similarity).
Associates with secondary piRNAs antisense and PIWIL2/MILI is required for such association (By similarity).
The piRNA process acts upstream of known mediators of DNA methylation (By similarity).
Does not show endonuclease activity (By similarity).
Plays a key role in the piRNA amplification loop, also named ping-pong amplification cycle, by acting as a 'slicer-incompetent' component that loads cleaved piRNAs from the 'slicer-competent' component PIWIL2 and target them on genomic transposon loci in the nucleus (By similarity).
May be involved in the chromatin-modifying pathway by inducing 'Lys-9' methylation of histone H3 at some loci (PubMed:17544373).
In addition to its role in germline, PIWIL4 also plays a role in the regulation of somatic cells activities. Plays a role in pancreatic beta cell function and insulin secretion (By similarity).
Involved in maintaining cell morphology and functional integrity of retinal epithelial through Akt/GSK3alpha/beta signaling pathway (PubMed:28025795).
When overexpressed, acts as an oncogene by inhibition of apoptosis and promotion of cells proliferation in tumors (PubMed:22483988).
Biological Process
Cell differentiationIEA:UniProtKB-KW
DNA methylation involved in gamete generationISS:UniProtKB
Epithelial structure maintenanceManual Assertion Based On ExperimentIMP:UniProtKB
Gene silencing by RNAISS:UniProtKB
Meiotic cell cycleIEA:UniProtKB-KW
Negative regulation of transpositionISS:UniProtKB
piRNA metabolic processISS:UniProtKB
Regulation of translationIEA:UniProtKB-KW
SpermatogenesisISS:UniProtKB
Cellular Location
Nucleus
Cytoplasm
Probable component of the meiotic nuage, also named P granule, a germ-cell-specific organelle required to repress transposon activity during meiosis. PIWIL2/MILI is required for nuclear localization (By similarity).
PTM
Arginine methylation by PRMT5 is required for the interaction with Tudor domain-containing protein (TDRD1, TDRKH/TDRD2 and TDRD9) and subsequent localization to the meiotic nuage, also named P granule.
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For research use only. Not intended for any clinical use.

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