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Mouse Anti-PLK4 Recombinant Antibody (1C8) (CBMAB-A6806-LY)


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Summary

Host Animal
Mouse
Specificity
Human
Clone
1C8
Antibody Isotype
IgG1, κ
Application
sELISA, ELISA

Basic Information

Immunogen
PLK4 (AAH36023, 1 a.a. ~ 110 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human
Antibody Isotype
IgG1, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
polo-like kinase 4 (Drosophila)
Introduction
This gene encodes a member of the polo family of serine/threonine protein kinases. The protein localizes to centrioles, complex microtubule-based structures found in centrosomes, and regulates centriole duplication during the cell cycle. [provided by RefSeq]
Entrez Gene ID
10733
UniProt ID
O00444
Alternative Names
SAK; STK18
Function
Serine/threonine-protein kinase that plays a central role in centriole duplication. Able to trigger procentriole formation on the surface of the parental centriole cylinder, leading to the recruitment of centriole biogenesis proteins such as SASS6, CENPJ/CPAP, CCP110, CEP135 and gamma-tubulin. When overexpressed, it is able to induce centrosome amplification through the simultaneous generation of multiple procentrioles adjoining each parental centriole during S phase. Phosphorylates 'Ser-151' of FBXW5 during the G1/S transition, leading to inhibit FBXW5 ability to ubiquitinate SASS6. Its central role in centriole replication suggests a possible role in tumorigenesis, centrosome aberrations being frequently observed in tumors. Also involved in deuterosome-mediated centriole amplification in multiciliated that can generate more than 100 centrioles. Also involved in trophoblast differentiation by phosphorylating HAND1, leading to disrupt the interaction between HAND1 and MDFIC and activate HAND1. Phosphorylates CDC25C and CHEK2. Required for the recruitment of STIL to the centriole and for STIL-mediated centriole amplification (PubMed:22020124).
Phosphorylates CEP131 at 'Ser-78' and PCM1 at 'Ser-372' which is essential for proper organization and integrity of centriolar satellites (PubMed:30804208).
Biological Process
Centriole replicationManual Assertion Based On ExperimentIDA:ComplexPortal
Cilium assemblyManual Assertion Based On ExperimentIMP:UniProtKB
De novo centriole assembly involved in multi-ciliated epithelial cell differentiationISS:UniProtKB
Mitotic cell cycleManual Assertion Based On ExperimentIBA:GO_Central
Positive regulation of centriole replicationManual Assertion Based On ExperimentIDA:UniProtKB
Protein phosphorylationManual Assertion Based On ExperimentIDA:UniProtKB
Regulation of cytokinesisManual Assertion Based On ExperimentIBA:GO_Central
Trophoblast giant cell differentiationISS:UniProtKB
Cellular Location
Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole
Nucleus, nucleolus
Cleavage furrow
Cytoplasm, cytoskeleton, microtubule organizing center, centrosome
Component of the deuterosome, a structure that promotes de novo centriole amplification in multiciliated cells that can generate more than 100 centrioles. Associates with centrioles throughout the cell cycle. According to PubMed:16244668, it is not present at cleavage furrows.
Involvement in disease
Microcephaly and chorioretinopathy, autosomal recessive, 2 (MCCRP2):
A severe disorder characterized by microcephaly, delayed psychomotor development, growth retardation with dwarfism, and ocular abnormalities.
PTM
Acetylation by KAT2A and KAT2B impairs kinase activity by shifting the kinase to an inactive conformation.
Ubiquitinated; leading to its degradation by the proteasome.
Tyrosine-phosphorylated by TEC.
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For research use only. Not intended for any clinical use.

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