Human Recombinant PLK4, Active protein, GST Tag (V2LY-0526-LY6238)

Name: Human Recombinant PLK4, Active protein, GST Tag
SKU: V2LY-0526-LY6238
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

Baculovirus-Insect Cells

Protein Species

Human

Tag

GST Tag

Protein Construction

This product is Human Recombinant PLK4, Active protein, GST Tag consist of Amino Acid: Full Length and predicts a molecular mass of 140 kDa.

Molecule Mass

140 kDa

Sequence

Amino Acid: Full Length

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

Batch dependent.

Endotoxin

Please contact us for more information.

Format

Liquid

Buffer

Tris, NaCl, DTT, Glycerol

Preservative

None

Storage

Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

More Infomation

Target

Full Name

polo-like kinase 4 (Drosophila)

Function

Serine/threonine-protein kinase that plays a central role in centriole duplication. Able to trigger procentriole formation on the surface of the parental centriole cylinder, leading to the recruitment of centriole biogenesis proteins such as SASS6, CENPJ/CPAP, CCP110, CEP135 and gamma-tubulin. When overexpressed, it is able to induce centrosome amplification through the simultaneous generation of multiple procentrioles adjoining each parental centriole during S phase. Phosphorylates 'Ser-151' of FBXW5 during the G1/S transition, leading to inhibit FBXW5 ability to ubiquitinate SASS6. Its central role in centriole replication suggests a possible role in tumorigenesis, centrosome aberrations being frequently observed in tumors. Also involved in deuterosome-mediated centriole amplification in multiciliated that can generate more than 100 centrioles. Also involved in trophoblast differentiation by phosphorylating HAND1, leading to disrupt the interaction between HAND1 and MDFIC and activate HAND1. Phosphorylates CDC25C and CHEK2. Required for the recruitment of STIL to the centriole and for STIL-mediated centriole amplification (PubMed:22020124).
Phosphorylates CEP131 at 'Ser-78' and PCM1 at 'Ser-372' which is essential for proper organization and integrity of centriolar satellites (PubMed:30804208).

Biological Process

Centriole replicationManual Assertion Based On ExperimentIDA:ComplexPortal
Cilium assemblyManual Assertion Based On ExperimentIMP:UniProtKB
De novo centriole assembly involved in multi-ciliated epithelial cell differentiationISS:UniProtKB
Mitotic cell cycleManual Assertion Based On ExperimentIBA:GO_Central
Positive regulation of centriole replicationManual Assertion Based On ExperimentIDA:UniProtKB
Protein phosphorylationManual Assertion Based On ExperimentIDA:UniProtKB
Regulation of cytokinesisManual Assertion Based On ExperimentIBA:GO_Central
Trophoblast giant cell differentiationISS:UniProtKB

Cellular Location

Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole
Nucleus, nucleolus
Cleavage furrow
Cytoplasm, cytoskeleton, microtubule organizing center, centrosome
Component of the deuterosome, a structure that promotes de novo centriole amplification in multiciliated cells that can generate more than 100 centrioles. Associates with centrioles throughout the cell cycle. According to PubMed:16244668, it is not present at cleavage furrows.

Involvement in disease

Microcephaly and chorioretinopathy, autosomal recessive, 2 (MCCRP2):
A severe disorder characterized by microcephaly, delayed psychomotor development, growth retardation with dwarfism, and ocular abnormalities.

PTM

Acetylation by KAT2A and KAT2B impairs kinase activity by shifting the kinase to an inactive conformation.
Ubiquitinated; leading to its degradation by the proteasome.
Tyrosine-phosphorylated by TEC.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human Recombinant PLK4 (G95L), Active protein, GST Tag (CAT#: V2LY-0526-LY6239)

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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