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Mouse Anti-PMEL Recombinant Antibody (NKI-beteb) (CBMAB-P2219-YC)

Provided herein is a Mouse monoclonal antibody against Human Premelanosome Protein. The antibody can be used for immunoassay techniques, such as WB, ELISA, IHC-P, IHC-Fr.
See all PMEL antibodies

Summary

Host Animal
Mouse
Specificity
Human, Horse
Clone
NKI-beteb
Antibody Isotype
IgG2b
Application
WB, ELISA, IHC-P, IHC-Fr

Basic Information

Immunogen
Lymph node pigmented melanoma metastases extract
Specificity
Human, Horse
Antibody Isotype
IgG2b
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Premelanosome protein
Introduction
PMEL is a melanocyte-specific type I transmembrane glycoprotein. The encoded protein is enriched in melanosomes, which are the melanin-producing organelles in melanocytes, and plays an essential role in the structural organization of premelanosomes. This protein is involved in generating internal matrix fibers that define the transition from Stage I to Stage II melanosomes. This protein undergoes a complex pattern of prosttranslational processing and modification that is essential to the proper functioning of the protein. A secreted form of this protein that is released by proteolytic ectodomain shedding may be used as a melanoma-specific serum marker.
Entrez Gene ID
Human6490
Horse100033885
UniProt ID
HumanP40967
HorseF7DCG1
Alternative Names
D12S53E; ME20; ME20-M; ME20M; P1; P100; PMEL17; SI; SIL; SILV; gp100
Function
Plays a central role in the biogenesis of melanosomes. Involved in the maturation of melanosomes from stage I to II. The transition from stage I melanosomes to stage II melanosomes involves an elongation of the vesicle, and the appearance within of distinct fibrillar structures. Release of the soluble form, ME20-S, could protect tumor cells from antibody mediated immunity.
Biological Process
Melanin biosynthetic processIEA:UniProtKB-KW
Melanosome organizationManual Assertion Based On ExperimentIDA:UniProtKB
Cellular Location
Endoplasmic reticulum membrane
Golgi apparatus
Melanosome
Endosome, multivesicular body
Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Localizes predominantly to intralumenal vesicles (ILVs) within multivesicular bodies. Associates with ILVs found within the lumen of premelanosomes and melanosomes and particularly in compartments that serve as precursors to the striated stage II premelanosomes.
M-alpha
Secreted
Topology
Lumenal: 470-595
Helical: 596-616
Cytoplasmic: 617-661
PTM
A small amount of P1/P100 (major form) undergoes glycosylation to yield P2/P120 (minor form). P2 is cleaved by a furin-like proprotein convertase (PC) in a pH-dependent manner in a post-Golgi, prelysosomal compartment into two disulfide-linked subunits: a large lumenal subunit, M-alpha/ME20-S, and an integral membrane subunit, M-beta. Despite cleavage, only a small fraction of M-alpha is secreted, whereas most M-alpha and M-beta remain associated with each other intracellularly. M-alpha is further processed to M-alpha N and M-alpha C. M-alpha C further undergoes processing to yield M-alpha C1 and M-alpha C3 (M-alpha C2 in the case of PMEL17-is or PMEL17-ls). Formation of intralumenal fibrils in the melanosomes requires the formation of M-alpha that becomes incorporated into the fibrils. Stage II melanosomes harbor only Golgi-modified Pmel17 fragments that are derived from M-alpha and that bear sialylated O-linked oligosaccharides.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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