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Mouse Anti-POLA2 Recombinant Antibody (2759C3a) (CBMAB-P2304-YC)

Provided herein is a Mouse monoclonal antibody against Human DNA Polymerase Alpha 2, Accessory Subunit. The antibody can be used for immunoassay techniques, such as Dot, ICC, WB.
See all POLA2 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
2759C3a
Antibody Isotype
IgG2b
Application
Dot, ICC, WB

Basic Information

Immunogen
Recombinant human Polymerase (Dna Directed), Alpha 2 (70Kd Subunit)Immunogen type:Recombinant protein
Specificity
Human
Antibody Isotype
IgG2b
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
POLA2
Introduction
POLA2 (DNA Polymerase Alpha 2, Accessory Subunit) is a protein coding gene. Among its related pathways are Telomere C-strand (Lagging Strand) Synthesis and Cell Cycle, Mitotic. Gene Ontology annotations related to this gene include protein heterodimerization activity and DNA-directed DNA polymerase activity.
Entrez Gene ID
23649
UniProt ID
Q14181
Alternative Names
Polymerase (DNA-Directed), Alpha (70kD); Polymerase (DNA Directed), Alpha 2 (70kD Subunit)
Function
Accessory subunit of the DNA polymerase alpha complex (also known as the alpha DNA polymerase-primase complex) which plays an essential role in the initiation of DNA synthesis (PubMed:9705292).
During the S phase of the cell cycle, the DNA polymerase alpha complex (composed of a catalytic subunit POLA1, an accessory subunit POLA2 and two primase subunits, the catalytic subunit PRIM1 and the regulatory subunit PRIM2) is recruited to DNA at the replicative forks via direct interactions with MCM10 and WDHD1 (By similarity).
The primase subunit of the polymerase alpha complex initiates DNA synthesis by oligomerising short RNA primers on both leading and lagging strands (By similarity).
These primers are initially extended by the polymerase alpha catalytic subunit and subsequently transferred to polymerase delta and polymerase epsilon for processive synthesis on the lagging and leading strand, respectively (By similarity).
Biological Process
DNA replication1 PublicationNAS:UniProtKB
DNA replication initiationManual Assertion Based On ExperimentIDA:ComplexPortal
DNA replication, synthesis of RNA primerManual Assertion Based On ExperimentIDA:UniProtKB
Protein import into nucleusIEA:Ensembl
Cellular Location
Nucleus
PTM
Phosphorylated in a cell cycle-dependent manner, in G2/M phase.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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