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Mouse Anti-POLA2 Recombinant Antibody (2F11) (CBMAB-A6856-LY)


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Summary

Host Animal
Mouse
Specificity
Human
Clone
2F11
Antibody Isotype
IgG1, κ
Application
WB, ELISA

Basic Information

Immunogen
POLA2 (AAH01347, 1 a.a. ~ 598 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human
Antibody Isotype
IgG1, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
POLA2
Entrez Gene ID
23649
UniProt ID
Q14181
Alternative Names
FLJ21662; FLJ37250
Function
Accessory subunit of the DNA polymerase alpha complex (also known as the alpha DNA polymerase-primase complex) which plays an essential role in the initiation of DNA synthesis (PubMed:9705292).
During the S phase of the cell cycle, the DNA polymerase alpha complex (composed of a catalytic subunit POLA1, an accessory subunit POLA2 and two primase subunits, the catalytic subunit PRIM1 and the regulatory subunit PRIM2) is recruited to DNA at the replicative forks via direct interactions with MCM10 and WDHD1 (By similarity).
The primase subunit of the polymerase alpha complex initiates DNA synthesis by oligomerising short RNA primers on both leading and lagging strands (By similarity).
These primers are initially extended by the polymerase alpha catalytic subunit and subsequently transferred to polymerase delta and polymerase epsilon for processive synthesis on the lagging and leading strand, respectively (By similarity).
Biological Process
DNA replication1 PublicationNAS:UniProtKB
DNA replication initiationManual Assertion Based On ExperimentIDA:ComplexPortal
DNA replication, synthesis of RNA primerManual Assertion Based On ExperimentIDA:UniProtKB
Protein import into nucleusIEA:Ensembl
Cellular Location
Nucleus
PTM
Phosphorylated in a cell cycle-dependent manner, in G2/M phase.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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