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Mouse Anti-POLB Recombinant Antibody (3H14) (CBMAB-D2169-YC)

Provided herein is a Mouse monoclonal antibody, which recognizes rat DNA Polymerase, beta. The antibody can be used for immunoassay techniques, such as ELISA, WB.
See all POLB antibodies

Summary

Host Animal
Mouse
Specificity
Human, Frog, Hamster, Mouse, Rat
Clone
3H14
Antibody Isotype
IgG
Application
ELISA, WB

Basic Information

Specificity
Human, Frog, Hamster, Mouse, Rat
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
DNA Polymerase Beta
Introduction
POLB is a DNA polymerase involved in base excision and repair, also called gap-filling DNA synthesis. POLB, acting as a monomer, is normally found in the cytoplasm, but it translocates to the nucleus upon DNA damage.
Entrez Gene ID
Human5423
Mouse18970
Rat29240
Hamster101834160
Frog448741
UniProt ID
HumanP06746
MouseQ8K409
RatP06766
HamsterG3HHH7
FrogO57383
Alternative Names
DNA Polymerase Beta; polymerase (DNA directed), beta
Function
Repair polymerase that plays a key role in base-excision repair. Has 5'-deoxyribose-5-phosphate lyase (dRP lyase) activity that removes the 5' sugar phosphate and also acts as a DNA polymerase that adds one nucleotide to the 3' end of the arising single-nucleotide gap. Conducts 'gap-filling' DNA synthesis in a stepwise distributive fashion rather than in a processive fashion as for other DNA polymerases.
Biological Process
AgingIEA:Ensembl
Base-excision repairManual Assertion Based On ExperimentIDA:UniProtKB
Base-excision repair, gap-fillingTAS:Reactome
Cellular response to DNA damage stimulusManual Assertion Based On ExperimentIMP:UniProtKB
DNA repairManual Assertion Based On ExperimentTAS:ProtInc
DNA-templated DNA replicationManual Assertion Based On ExperimentTAS:ProtInc
Double-strand break repair via nonhomologous end joiningManual Assertion Based On ExperimentIBA:GO_Central
Homeostasis of number of cellsIEA:Ensembl
Immunoglobulin heavy chain V-D-J recombinationIEA:Ensembl
In utero embryonic developmentIEA:Ensembl
Inflammatory responseIEA:Ensembl
Intrinsic apoptotic signaling pathway in response to DNA damageIEA:Ensembl
Lymph node developmentIEA:Ensembl
Neuron apoptotic processIEA:Ensembl
Pyrimidine dimer repairIEA:Ensembl
Response to ethanolIEA:Ensembl
Response to gamma radiationIEA:Ensembl
Response to hyperoxiaIEA:Ensembl
Salivary gland morphogenesisIEA:Ensembl
Somatic hypermutation of immunoglobulin genesIEA:Ensembl
Spleen developmentIEA:Ensembl
Cellular Location
Nucleus
Cytoplasm
Cytoplasmic in normal conditions. Translocates to the nucleus following DNA damage.
PTM
Methylation by PRMT6 stimulates the polymerase activity by enhancing DNA binding and processivity.
Ubiquitinated at Lys-41, Lys-61 and Lys-81: monoubiquitinated by HUWE1/ARF-BP1. Monoubiquitinated protein is then the target of STUB1/CHIP, which catalyzes polyubiquitination from monoubiquitin, leading to degradation by the proteasome. USP47 mediates the deubiquitination of monoubiquitinated protein, preventing polyubiquitination by STUB1/CHIP and its subsequent degradation.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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