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Mouse Anti-POLE Recombinant Antibody (3C133) (CBMAB-D2179-YC)

Provided herein is a Mouse monoclonal antibody, which specific to the human DNA pol epsilon and does not recognize DNA pol alpha, beta or delta. The antibody can be used for immunoassay techniques, such as ELISA.
See all POLE antibodies

Summary

Host Animal
Mouse
Specificity
Human, Hamster, Monkey, Mouse
Clone
3C133
Antibody Isotype
IgG2a
Application
ELISA

Basic Information

Specificity
Human, Hamster, Monkey, Mouse
Antibody Isotype
IgG2a
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
DNA Polymerase Epsilon, Catalytic Subunit
Introduction
POLE is the catalytic subunit of DNA polymerase epsilon. POLE is involved in DNA repair and chromosomal DNA replication. Mutations in POLE have been associated with colorectal cancer 12 and facial dysmorphism, immunodeficiency, livedo, and short stature.
Entrez Gene ID
Human5426
Mouse18973
Hamster100774671
Monkey698023
UniProt ID
HumanQ07864
MouseQ9WVF7
MonkeyF6QAQ1
Alternative Names
FILS; POLE1; CRCS12
Function
Catalytic component of the DNA polymerase epsilon complex (PubMed:10801849).
Participates in chromosomal DNA replication (By similarity).
Required during synthesis of the leading DNA strands at the replication fork, binds at/or near replication origins and moves along DNA with the replication fork (By similarity).
Has 3'-5' proofreading exonuclease activity that corrects errors arising during DNA replication (By similarity).
Involved in DNA synthesis during DNA repair (PubMed:20227374, PubMed:27573199).
Along with DNA polymerase POLD1 and DNA polymerase POLK, has a role in excision repair (NER) synthesis following UV irradiation (PubMed:20227374).
Biological Process
Base-excision repair, gap-fillingManual Assertion Based On ExperimentIDA:UniProtKB
DNA replicationManual Assertion Based On ExperimentIMP:UniProtKB
DNA replication proofreadingManual Assertion Based On ExperimentIBA:GO_Central
DNA synthesis involved in DNA repairManual Assertion Based On ExperimentIMP:UniProtKB
DNA-templated DNA replicationManual Assertion Based On ExperimentIDA:ComplexPortal
Embryonic organ developmentIEA:Ensembl
G1/S transition of mitotic cell cycleManual Assertion Based On ExperimentIMP:UniProtKB
Leading strand elongationManual Assertion Based On ExperimentIBA:GO_Central
Mitotic cell cycleManual Assertion Based On ExperimentIBA:GO_Central
Nucleotide-excision repair, DNA gap fillingManual Assertion Based On ExperimentIMP:UniProtKB
Cellular Location
Nucleus
Involvement in disease
Colorectal cancer 12 (CRCS12):
A complex disease characterized by malignant lesions arising from the inner wall of the large intestine (the colon) and the rectum. Genetic alterations are often associated with progression from premalignant lesion (adenoma) to invasive adenocarcinoma. Risk factors for cancer of the colon and rectum include colon polyps, long-standing ulcerative colitis, and genetic family history. CRCS12 is characterized by a high-penetrance predisposition to the development of colorectal adenomas and carcinomas, with a variable tendency to develop multiple and large tumors. Onset is usually before age 40 years. The histologic features of the tumors are unremarkable.
Facial dysmorphism, immunodeficiency, livedo, and short stature (FILS):
A syndrome characterized by mild facial dysmorphism, mainly malar hypoplasia, livedo on the skin since birth, and immunodeficiency resulting in recurrent infections. Growth impairment is observed during early childhood and results in variable short stature in adulthood.
Intrauterine growth retardation, metaphyseal dysplasia, adrenal hypoplasia congenita, genital anomalies, and immunodeficiency (IMAGEI):
An autosomal recessive disorder characterized by intrauterine growth retardation, postnatal growth failure, metaphyseal dysplasia, adrenal hypoplasia congenita, growth hormone deficiency, genital anomalies, and immunodeficiency resulting in increased infections.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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