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Mouse Anti-POLE Recombinant Antibody (CBYC-P496) (CBMAB-P2321-YC)

Provided herein is a Mouse monoclonal antibody against Human DNA Polymerase Epsilon, Catalytic Subunit. The antibody can be used for immunoassay techniques, such as ELISA, IP, WB.
See all POLE antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
CBYC-P496
Antibody Isotype
IgG1, κ
Application
ELISA, IP, WB

Basic Information

Specificity
Human
Antibody Isotype
IgG1, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
PBS, pH 7.2
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
DNA Polymerase Epsilon, Catalytic Subunit
Introduction
POLE (DNA Polymerase Epsilon, Catalytic Subunit) is a protein coding gene. Diseases associated with POLE include Facial Dysmorphism, Immunodeficiency, Livedo, And Short Stature and Colorectal Cancer 12. Among its related pathways are Telomere C-strand (Lagging Strand) Synthesis and Cell Cycle, Mitotic. Gene Ontology annotations related to this gene include nucleic acid binding and chromatin binding.
Entrez Gene ID
5426
UniProt ID
Q07864
Alternative Names
CRCS12; FILS; POLE1
Function
Catalytic component of the DNA polymerase epsilon complex (PubMed:10801849).
Participates in chromosomal DNA replication (By similarity).
Required during synthesis of the leading DNA strands at the replication fork, binds at/or near replication origins and moves along DNA with the replication fork (By similarity).
Has 3'-5' proofreading exonuclease activity that corrects errors arising during DNA replication (By similarity).
Involved in DNA synthesis during DNA repair (PubMed:20227374, PubMed:27573199).
Along with DNA polymerase POLD1 and DNA polymerase POLK, has a role in excision repair (NER) synthesis following UV irradiation (PubMed:20227374).
Biological Process
Base-excision repair, gap-fillingManual Assertion Based On ExperimentIDA:UniProtKB
DNA replicationManual Assertion Based On ExperimentIMP:UniProtKB
DNA replication proofreadingManual Assertion Based On ExperimentIBA:GO_Central
DNA synthesis involved in DNA repairManual Assertion Based On ExperimentIMP:UniProtKB
DNA-templated DNA replicationManual Assertion Based On ExperimentIDA:ComplexPortal
Embryonic organ developmentIEA:Ensembl
G1/S transition of mitotic cell cycleManual Assertion Based On ExperimentIMP:UniProtKB
Leading strand elongationManual Assertion Based On ExperimentIBA:GO_Central
Mitotic cell cycleManual Assertion Based On ExperimentIBA:GO_Central
Nucleotide-excision repair, DNA gap fillingManual Assertion Based On ExperimentIMP:UniProtKB
Cellular Location
Nucleus
Involvement in disease
Colorectal cancer 12 (CRCS12):
A complex disease characterized by malignant lesions arising from the inner wall of the large intestine (the colon) and the rectum. Genetic alterations are often associated with progression from premalignant lesion (adenoma) to invasive adenocarcinoma. Risk factors for cancer of the colon and rectum include colon polyps, long-standing ulcerative colitis, and genetic family history. CRCS12 is characterized by a high-penetrance predisposition to the development of colorectal adenomas and carcinomas, with a variable tendency to develop multiple and large tumors. Onset is usually before age 40 years. The histologic features of the tumors are unremarkable.
Facial dysmorphism, immunodeficiency, livedo, and short stature (FILS):
A syndrome characterized by mild facial dysmorphism, mainly malar hypoplasia, livedo on the skin since birth, and immunodeficiency resulting in recurrent infections. Growth impairment is observed during early childhood and results in variable short stature in adulthood.
Intrauterine growth retardation, metaphyseal dysplasia, adrenal hypoplasia congenita, genital anomalies, and immunodeficiency (IMAGEI):
An autosomal recessive disorder characterized by intrauterine growth retardation, postnatal growth failure, metaphyseal dysplasia, adrenal hypoplasia congenita, growth hormone deficiency, genital anomalies, and immunodeficiency resulting in increased infections.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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