Mouse Anti-POLR2I Recombinant Antibody (2B3) (CBMAB-P2360-YC)

Name: Mouse Anti-POLR2I Recombinant Antibody (2B3)
SKU: CBMAB-P2360-YC
Rating: 5 (1 reviews)

See all POLR2I antibodies

Compare Online Inquiry

Datasheet

SDS

Request for COA

Datasheet Target Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

2B3

Application

ELISA, WB

Immunogen

POLR2I (AAH17112, AA 26-125 partial recombinant protein with GST tag.

Specificity

Human

Antibody Isotype

IgG1, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

polymerase (RNA) II (DNA directed) polypeptide I, 14.5kDa

Introduction

POLR2I is a subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit, in combination with two other polymerase subunits, forms the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA. The product of this gene has two zinc finger motifs with conserved cysteines and the subunit does possess zinc binding activity.

Entrez Gene ID

5438

UniProt ID

P36954

Alternative Names

hRPB14.5; RPB9

Function

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB9 is part of the upper jaw surrounding the central large cleft and thought to grab the incoming DNA template (By similarity).

Biological Process

Maintenance of transcriptional fidelity during DNA-templated transcription elongation from RNA polymerase II promoterManual Assertion Based On ExperimentIBA:GO_Central
mRNA cleavageIEA:InterPro
Transcription by RNA polymerase IIManual Assertion Based On ExperimentIDA:UniProtKB
Transcription initiation from RNA polymerase II promoterManual Assertion Based On ExperimentIBA:GO_Central
Transcription-coupled nucleotide-excision repairManual Assertion Based On ExperimentIBA:GO_Central

Cellular Location

Nucleus, nucleolus

Ask a question We look forward to hearing from you.

0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Have you used Mouse Anti-POLR2I Recombinant Antibody (2B3)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon

$30 eGift Card
Submit a review

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Related Products

Mouse Anti-POLR2I Recombinant Antibody (2A7) (CAT#: CBMAB-R0648-CN)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Request bulk or custom quote Second antibody and isotype control

Contact us

Tel: (USA)
(UK)
Fax:

Global Locations

Email:

Online Inquiry