Mouse Anti-POLRMT Recombinant Antibody (2H7) (CBMAB-P2380-YC)

Name: Mouse Anti-POLRMT Recombinant Antibody (2H7)
SKU: CBMAB-P2380-YC
Rating: 5 (1 reviews)

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Datasheet

SDS

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Basic Information

Host Animal

Mouse

Clone

2H7

Application

ELISA, WB

Immunogen

POLRMT (NP_005026.2, 1-100 aa) partial recombinant protein with GST tag. Immunogen sequence: MSALCWGRGA AGLKRALRPC GRPGLPGKEG TAGGVCGPRR SSSASPQEQD QDRRKDWGHV ELLEVLQARV RQLQAESVSE VVVNRVDVAR LPECGSGDGS

Specificity

Human

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.

Epitope

aa 1-100

More Infomation

Target

Full Name

polymerase (RNA) mitochondrial (DNA directed)

Introduction

POLRMT is a mitochondrial DNA-directed RNA polymerase. The gene product is responsible for mitochondrial gene expression as well as for providing RNA primers for initiation of replication of the mitochondrial genome. Although this polypeptide has the same function as the three nuclear DNA-directed RNA polymerases, it is more closely related to RNA polymerases of phage and mitochondrial polymerases of lower eukaryotes.

Entrez Gene ID

5442

UniProt ID

O00411

Alternative Names

MTRNAP; h-mtRPOL; MTRPOL; APOLMT

Function

DNA-dependent RNA polymerase catalyzes the transcription of mitochondrial DNA into RNA using the four ribonucleoside triphosphates as substrates (PubMed:21278163).
Component of the mitochondrial transcription initiation complex, composed at least of TFB2M, TFAM and POLRMT that is required for basal transcription of mitochondrial DNA (PubMed:29149603).
In this complex, TFAM recruits POLRMT to a specific promoter whereas TFB2M induces structural changes in POLRMT to enable promoter opening and trapping of the DNA non-template strand (PubMed:29149603).

Biological Process

Mitochondrial transcriptionManual Assertion Based On ExperimentIDA:UniProtKB
Transcription initiation from mitochondrial promoterManual Assertion Based On ExperimentIDA:ComplexPortal

Cellular Location

Mitochondrion

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Related Products

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Mouse Anti-POLRMT Recombinant Antibody (3D9) (CAT#: CBMAB-P2381-YC)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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