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Mouse Anti-POLRMT Recombinant Antibody (3D9) (CBMAB-P2381-YC)


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Summary

Host Animal
Mouse
Specificity
Human
Clone
3D9
Antibody Isotype
IgG2a, κ
Application
ELISA, IF

Basic Information

Immunogen
POLRMT (NP_005026, 1121-1230 aa) partial recombinant protein with GST tag. Immunogen sequence: PNFIHSLDSS HMMLTALHCY RKGLTFVSVH DCYWTHAADV SVMNQVCREQ FVRLHSEPIL QDLSRFLVKR FCSEPQKILE ASQLKETLQA VPKPGAFDLE QVKRSTYFFS
Specificity
Human
Antibody Isotype
IgG2a, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.
Epitope
aa 1121-1230

Target

Full Name
polymerase (RNA) mitochondrial (DNA directed)
Introduction
POLRMT is a mitochondrial DNA-directed RNA polymerase. The gene product is responsible for mitochondrial gene expression as well as for providing RNA primers for initiation of replication of the mitochondrial genome. Although this polypeptide has the same function as the three nuclear DNA-directed RNA polymerases, it is more closely related to RNA polymerases of phage and mitochondrial polymerases of lower eukaryotes.
Entrez Gene ID
5442
UniProt ID
O00411
Alternative Names
MTRNAP; h-mtRPOL; MTRPOL; APOLMT
Function
DNA-dependent RNA polymerase catalyzes the transcription of mitochondrial DNA into RNA using the four ribonucleoside triphosphates as substrates (PubMed:21278163).
Component of the mitochondrial transcription initiation complex, composed at least of TFB2M, TFAM and POLRMT that is required for basal transcription of mitochondrial DNA (PubMed:29149603).
In this complex, TFAM recruits POLRMT to a specific promoter whereas TFB2M induces structural changes in POLRMT to enable promoter opening and trapping of the DNA non-template strand (PubMed:29149603).
Biological Process
Mitochondrial transcriptionManual Assertion Based On ExperimentIDA:UniProtKB
Transcription initiation from mitochondrial promoterManual Assertion Based On ExperimentIDA:ComplexPortal
Cellular Location
Mitochondrion
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For research use only. Not intended for any clinical use.

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