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Mouse Anti-PPP1CC Recombinant Antibody (AF11F10) (CBMAB-P2592-YC)

Provided herein is a Mouse monoclonal antibody against Human Protein Phosphatase 1 Catalytic Subunit Gamma. The antibody can be used for immunoassay techniques, such as ELISA, WB.
See all PPP1CC antibodies

Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
AF11F10
Antibody Isotype
IgG2a
Application
ELISA, WB

Basic Information

Immunogen
Recombinant human protein purified from E. coli (His-PP1G)
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG2a
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
HEPES with 0.01% BSA, 150 mM sodium chloride, 50% glycerol and 0.03% sodium azide
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Protein Phosphatase 1 Catalytic Subunit Gamma
Introduction
PPP1CC belongs to the protein phosphatase family, PP1 subfamily. PP1 is an ubiquitous serine/threonine phosphatase that regulates many cellular processes, including cell division. It is expressed in mammalian cells as three closely related isoforms, alpha, beta/delta and gamma, which have distinct localization patterns. This gene encodes the gamma isozyme.
Entrez Gene ID
Human5501
Mouse19047
Rat24669
UniProt ID
HumanP36873
MouseP63087
RatP63088
Alternative Names
PP-1G; PPP1G; dis2m1; PP1; PP1C; Ppp1cc1
Function
Protein phosphatase that associates with over 200 regulatory proteins to form highly specific holoenzymes which dephosphorylate hundreds of biological targets. Protein phosphatase 1 (PP1) is essential for cell division, and participates in the regulation of glycogen metabolism, muscle contractility and protein synthesis. Dephosphorylates RPS6KB1. Involved in regulation of ionic conductances and long-term synaptic plasticity. May play an important role in dephosphorylating substrates such as the postsynaptic density-associated Ca2+/calmodulin dependent protein kinase II. Component of the PTW/PP1 phosphatase complex, which plays a role in the control of chromatin structure and cell cycle progression during the transition from mitosis into interphase. In balance with CSNK1D and CSNK1E, determines the circadian period length, through the regulation of the speed and rhythmicity of PER1 and PER2 phosphorylation. May dephosphorylate CSNK1D and CSNK1E. Dephosphorylates the 'Ser-418' residue of FOXP3 in regulatory T-cells (Treg) from patients with rheumatoid arthritis, thereby inactivating FOXP3 and rendering Treg cells functionally defective (PubMed:23396208).
Biological Process
Cell cycleIEA:UniProtKB-KW
Cell divisionIEA:UniProtKB-KW
Circadian regulation of gene expressionISS:UniProtKB
Entrainment of circadian clock by photoperiodISS:UniProtKB
Glycogen metabolic processIEA:UniProtKB-KW
MAPK cascadeTAS:Reactome
Neuron differentiationIEA:Ensembl
Positive regulation of glial cell proliferationIEA:Ensembl
Protein dephosphorylationManual Assertion Based On ExperimentIMP:MGI
Regulation of circadian rhythmManual Assertion Based On ExperimentIMP:UniProtKB
Regulation of nucleocytoplasmic transportIEA:Ensembl
Cellular Location
Cytoplasm
Nucleus
Nucleus, nucleolus
Nucleus, nucleoplasm
Nucleus speckle
Chromosome, centromere, kinetochore
Cleavage furrow
Midbody
Mitochondrion
Cytoplasm, cytoskeleton, microtubule organizing center
Colocalizes with SPZ1 in the nucleus (By similarity).
Colocalizes with URI1 at mitochondrion (PubMed:17936702).
Rapidly exchanges between the nucleolar, nucleoplasmic and cytoplasmic compartments (PubMed:11739654).
Highly mobile in cells and can be relocalized through interaction with targeting subunits (PubMed:17965019).
In the presence of PPP1R8 relocalizes from the nucleolus to nuclear speckles (PubMed:11739654).
Shows a dynamic targeting to specific sites throughout the cell cycle (PubMed:12529430).
Highly concentrated in nucleoli of interphase cells and localizes at kinetochores early in mitosis (PubMed:12529430).
Relocalization to chromosome-containing regions occurs at the transition from early to late anaphase (PubMed:12529430).
Also accumulates at the cleavage furrow and midbody by telophase (PubMed:12529430).
Colocalizes with DYNLT4 in the microtubule organizing center (MTOC)(PubMed:23789093).
PTM
Phosphorylated by NEK2.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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