Rabbit Anti-PRDX3 Recombinant Antibody (
14E2) (V2LY-0725-LY1206)

Name: Rabbit Anti-PRDX3 Recombinant Antibody (14E2)
SKU: V2LY-0725-LY1206
Rating: 5 (1 reviews)

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Tested Data

Rabbit Anti-PRDX3 Recombinant Antibody (14E2) (V2LY-0725-LY1206) in WB

Western Blot
Positive WB detected in: PC3 whole cell lysate, Ntera-2 whole cell lysate, HepG2 whole cell lysate, A549 whole cell lysate
All lanes: PRDX3 antibody at 1:2000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 28, 26 kDa
Observed band size: 20-28 kDa

Rabbit Anti-PRDX3 Recombinant Antibody (14E2) (V2LY-0725-LY1206) in IHC

IHC image of V2LY-0725-LY1206 diluted at 1:100 and staining in paraffin-embedded human lung tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.

Rabbit Anti-PRDX3 Recombinant Antibody (14E2) (V2LY-0725-LY1206) in IHC

IHC image of V2LY-0725-LY1206 diluted at 1:100 and staining in paraffin-embedded human stomach tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.

Rabbit Anti-PRDX3 Recombinant Antibody (14E2) (V2LY-0725-LY1206) in IF

Immunofluorescence staining of PC-3 cell with V2LY-0725-LY1206 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 503-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Rabbit Anti-PRDX3 Recombinant Antibody (14E2) (V2LY-0725-LY1206) in FC

Overlay Peak curve showing HepG2 cells stained with V2LY-0725-LY1206 (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4°C.Control antibody (green line) was rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of>10,000 events was performed.

Datasheet

SDS

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Basic Information

Host Animal

Rabbit

Clone

14E2

Application

ELISA, WB, IHC, IF, FC

Immunogen

A synthesized peptide derived from human PRDX3.

Host Species

Rabbit

Specificity

Human

Antibody Isotype

IgG

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:500-1:2,000
IHC	1:50-1:200
IF	1:50-1:200
FC	1:50-1:200

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, glycerol

Preservative

Sodium azide

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

PRDX3

Entrez Gene ID

10935

UniProt ID

P30048

Function

Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively. Plays a role in cell protection against oxidative stress by detoxifying peroxides (PubMed:7733872, PubMed:17707404).
Acts synergistically with MAP3K13 to regulate the activation of NF-kappa-B in the cytosol (PubMed:12492477).

Biological Process

Cell redox homeostasisManual Assertion Based On ExperimentIBA:GO_Central
Cellular response to oxidative stressManual Assertion Based On ExperimentIDA:ParkinsonsUK-UCL
Cellular response to reactive oxygen speciesManual Assertion Based On ExperimentIMP:UniProtKB
Hydrogen peroxide catabolic processManual Assertion Based On ExperimentIDA:MGI
Maternal placenta developmentIEA:Ensembl
Mitochondrion organizationManual Assertion Based On ExperimentIMP:UniProtKB
Myeloid cell differentiationISS:UniProtKB
Negative regulation of apoptotic processManual Assertion Based On ExperimentIDA:ParkinsonsUK-UCL
Negative regulation of kinase activityManual Assertion Based On ExperimentIDA:UniProtKB
Peptidyl-cysteine oxidationManual Assertion Based On ExperimentIDA:ParkinsonsUK-UCL
Positive regulation of cell population proliferationManual Assertion Based On ExperimentIDA:UniProtKB
Positive regulation of NF-kappaB transcription factor activityManual Assertion Based On ExperimentIDA:UniProtKB
Regulation of mitochondrial membrane potentialManual Assertion Based On ExperimentIMP:UniProtKB
Response to hydrogen peroxideManual Assertion Based On ExperimentIDA:UniProtKB
Response to lipopolysaccharideISS:UniProtKB
Response to oxidative stressManual Assertion Based On ExperimentIMP:UniProtKB

Cellular Location

Mitochondrion
Cytoplasm
Early endosome
Localizes to early endosomes in a RPS6KC1-dependent manner.

PTM

Phosphorylated by LRRK2; phosphorylation reduces perodixase activity.
The enzyme can be inactivated by further oxidation of the cysteine sulfenic acid (C(P)-SOH) to sulphinic acid (C(P)-SO2H) and sulphonic acid (C(P)-SO3H) instead of its condensation to a disulfide bond.
S-palmitoylated.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Antibody Pairs

PRDX3 Matched Antibody Pair (924) (CAT#: APMAB-924LY)

PRDX3 Matched Antibody Pair (925) (CAT#: APMAB-925LY)

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Isotype control

For research use only. Not intended for any clinical use.

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