Mouse Anti-PRDX6 Recombinant Antibody (4A3) (CBMAB-P2750-YC)

Name: Mouse Anti-PRDX6 Recombinant Antibody (4A3)
SKU: CBMAB-P2750-YC
Rating: 5 (1 reviews)

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Datasheet

SDS

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Basic Information

Host Animal

Mouse

Clone

4A3

Application

WB, ELISA

Immunogen

Recombinant human protein purified from E. coli

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG1

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

HEPES with 150 mM sodium chloride, 0.01% BSA, 0.03% sodium azide, and 50% glycerol

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

peroxiredoxin 6

Introduction

PRDX6 is a member of the thiol-specific antioxidant protein family. This protein is a bifunctional enzyme with two distinct active sites. It is involved in redox regulation of the cell; it can reduce H(2)O(2) and short chain organic, fatty acid, and phospholipid hydroperoxides. It may play a role in the regulation of phospholipid turnover as well as in protection against oxidative injury.

Entrez Gene ID

Human	9588
Mouse	11758
Rat	94167

UniProt ID

Human	P30041
Mouse	O08709
Rat	O35244

Alternative Names

1-Cys, NSGPx, AOP2, aiPLA2, PRX; p29; AOP2; 1-Cys; NSGPx; aiPLA2; HEL-S-128m; Peroxiredoxin-6; 1-Cys peroxiredoxin; 1-Cys PRX; 24 kDa protein; Acidic calcium-independent phospholipase A2; Antioxidant protein 2; Liver 2D page spot 40; Non-selenium glutathione peroxidase; Red blood cells page spot 12; PRDX6; KIAA0106, p29, PRX, HEL-S-128m

Function

Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively (PubMed:9497358, PubMed:10893423).
Can reduce H2O2 and short chain organic, fatty acid, and phospholipid hydroperoxides (PubMed:10893423).
Also has phospholipase activity, can therefore either reduce the oxidized sn-2 fatty acyl group of phospholipids (peroxidase activity) or hydrolyze the sn-2 ester bond of phospholipids (phospholipase activity) (PubMed:10893423, PubMed:26830860).
These activities are dependent on binding to phospholipids at acidic pH and to oxidized phospholipds at cytosolic pH (PubMed:10893423).
Plays a role in cell protection against oxidative stress by detoxifying peroxides and in phospholipid homeostasis (PubMed:10893423).
Exhibits acyl-CoA-dependent lysophospholipid acyltransferase which mediates the conversion of lysophosphatidylcholine (1-acyl-sn-glycero-3-phosphocholine or LPC) into phosphatidylcholine (1,2-diacyl-sn-glycero-3-phosphocholine or PC) (PubMed:26830860).
Shows a clear preference for LPC as the lysophospholipid and for palmitoyl CoA as the fatty acyl substrate (PubMed:26830860).

Biological Process

Cell redox homeostasisManual Assertion Based On ExperimentIBA:GO_Central
Cellular oxidant detoxificationManual Assertion Based On ExperimentIMP:CAFA
Glycerophospholipid catabolic processManual Assertion Based On ExperimentIMP:CAFA
Positive regulation of mRNA splicing, via spliceosomeManual Assertion Based On ExperimentIGI:UniProtKB
Response to oxidative stressManual Assertion Based On ExperimentIMP:CAFA

Cellular Location

Cytoplasm
Lysosome
Also found in lung secretory organelles (lamellar bodies).

PTM

Irreversibly inactivated by overoxidation of Cys-47 to sulfinic acid (Cys-SO2H) and sulfonic acid (Cys-SO3H) forms upon oxidative stress.
Phosphorylation at Thr-177 by MAP kinases increases the phospholipase activity of the enzyme (By similarity).
The phosphorylated form exhibits a greater lysophosphatidylcholine acyltransferase activity compared to the non-phosphorylated form (PubMed:26830860).

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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