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Mouse Anti-PRDX6 Recombinant Antibody (6I8) (CBMAB-BR400LY)

The product is antibody recognizes PRDX6. The antibody 6I8 immunoassay techniques such as: FC, IF, IHC-P, ICC, WB.
See all PRDX6 antibodies

Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
6I8
Antibody Isotype
IgG2B
Application
FC: 1-3 μg/1x10 cells, IF: 1:1000 dilution, IHC-P: 0.5-1 μg/ml, ICC: 1:200 dilution, WB: 0.1-0.5 μg/ml

Basic Information

Immunogen
E.coli-derived human Peroxiredoxin 6 recombinant protein (Position: E15-P224).
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG2B
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Lyophilized
Preservative
0.05 mg sodium azide
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
peroxiredoxin 6
Entrez Gene ID
Human9588
Mouse11758
Rat94167
UniProt ID
HumanP30041
MouseO08709
RatO35244
Alternative Names
Peroxiredoxin-6;1.11.1.15;1-Cys peroxiredoxin;1-Cys PRX;24 kDa protein;Acidic calcium-independent phospholipase A2;aiPLA2;3.1.1.-;Antioxidant protein 2;Liver 2D page spot 40;Non-selenium glutathione peroxidase;NSGPx;1.11.1.9;Red blood cells page spot 12;PRDX6;AOP2, KIAA0106;
Function
Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively (PubMed:9497358, PubMed:10893423).
Can reduce H2O2 and short chain organic, fatty acid, and phospholipid hydroperoxides (PubMed:10893423).
Also has phospholipase activity, can therefore either reduce the oxidized sn-2 fatty acyl group of phospholipids (peroxidase activity) or hydrolyze the sn-2 ester bond of phospholipids (phospholipase activity) (PubMed:10893423, PubMed:26830860).
These activities are dependent on binding to phospholipids at acidic pH and to oxidized phospholipds at cytosolic pH (PubMed:10893423).
Plays a role in cell protection against oxidative stress by detoxifying peroxides and in phospholipid homeostasis (PubMed:10893423).
Exhibits acyl-CoA-dependent lysophospholipid acyltransferase which mediates the conversion of lysophosphatidylcholine (1-acyl-sn-glycero-3-phosphocholine or LPC) into phosphatidylcholine (1,2-diacyl-sn-glycero-3-phosphocholine or PC) (PubMed:26830860).
Shows a clear preference for LPC as the lysophospholipid and for palmitoyl CoA as the fatty acyl substrate (PubMed:26830860).
Biological Process
Cell redox homeostasisManual Assertion Based On ExperimentIBA:GO_Central
Cellular oxidant detoxificationManual Assertion Based On ExperimentIMP:CAFA
Glycerophospholipid catabolic processManual Assertion Based On ExperimentIMP:CAFA
Positive regulation of mRNA splicing, via spliceosomeManual Assertion Based On ExperimentIGI:UniProtKB
Response to oxidative stressManual Assertion Based On ExperimentIMP:CAFA
Cellular Location
Cytoplasm
Lysosome
Also found in lung secretory organelles (lamellar bodies).
PTM
Irreversibly inactivated by overoxidation of Cys-47 to sulfinic acid (Cys-SO2H) and sulfonic acid (Cys-SO3H) forms upon oxidative stress.
Phosphorylation at Thr-177 by MAP kinases increases the phospholipase activity of the enzyme (By similarity).
The phosphorylated form exhibits a greater lysophosphatidylcholine acyltransferase activity compared to the non-phosphorylated form (PubMed:26830860).
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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