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Mouse Anti-PRKD3 Recombinant Antibody (4G7) (CBMAB-A7054-LY)

The product is antibody recognizes PRKD3. The antibody 4G7 immunoassay techniques such as: WB, ELISA.
See all PRKD3 antibodies
Published Data
Fig. 1 The ER+ cell line T47D was treated with 100 nmol/L fulvestrant or DMSO (control) for 24 hours and cell lysates were analyzed by Western blot analysis for the expression of PKD3 or β-actin.
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Fig. 2 TMA slide containing human TNBC breast tissue samples was analyzed for PKD3 expression using anti-PKD3 antibody.
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Fig. 3 Lysates from treated cells were analyzed by Western blotting for expression of PKD3 or β-actin as a loading control.
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Summary

Host Animal
Mouse
Specificity
Human
Clone
4G7
Antibody Isotype
IgG2a, κ
Application
WB, ELISA

Basic Information

Immunogen
PRKD3 (AAH30706, 1 a.a. ~ 100 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human
Antibody Isotype
IgG2a, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
protein kinase D3
Introduction
Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role. The protein encoded by this gene is one of the PKC family members. This kinase can be activated rapidly by the agonists of G protein-coupled receptors. It resides in both cytoplasm and nucleus, and its nuclear accumulation is found to be dramatically enhanced in response to its activation. This kinase can also be activated after B-cell antigen receptor (BCR) engagement, which requires intact phopholipase C gamma and the involvement of other PKC family members. [provided by RefSeq]
Entrez Gene ID
23683
UniProt ID
O94806
Alternative Names
EPK2; PKC-NU; PKD3; PRKCN; nPKC-NU
Function
Converts transient diacylglycerol (DAG) signals into prolonged physiological effects, downstream of PKC. Involved in resistance to oxidative stress (By similarity).
Biological Process
Protein kinase C-activating G protein-coupled receptor signaling pathwayManual Assertion Based On ExperimentTAS:ProtInc
Protein kinase D signalingManual Assertion Based On ExperimentIBA:GO_Central
Protein phosphorylationManual Assertion Based On ExperimentTAS:ProtInc
Sphingolipid biosynthetic processTAS:Reactome
Cellular Location
Cytoplasm
Membrane
Translocation to the cell membrane is required for kinase activation.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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