Sign in or Register   Sign in or Register
  |  

Mouse Anti-PRPF8 Recombinant Antibody (2G8) (CBMAB-R0658-CN)

This product is a Mouse antibody that recognizes PRPF8. The antibody 2G8 can be used for immunoassay techniques such as: IF, WB.
See all PRPF8 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
2G8
Antibody Isotype
IgG2b, κ
Application
IF, WB

Basic Information

Immunogen
RP13-297E16.1 (NP_005079, aa. 53-157) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K
Specificity
Human
Antibody Isotype
IgG2b, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Target

Full Name
Pre-mRNA processing factor 8
Introduction
Pre-mRNA splicing occurs in 2 sequential transesterification steps. The protein encoded by this gene is a component of both U2- and U12-dependent spliceosomes, and found to be essential for the catalytic step II in pre-mRNA splicing process. It contains several WD repeats, which function in protein-protein interactions. The protein has a sequence similarity to yeast Prp8 protein. This gene is a candidate gene for autosomal dominant retinitis pigmentosa. [provided by RefSeq, Jul 2008]
Entrez Gene ID
10594
UniProt ID
Q6P2Q9
Alternative Names
PRP8; RP13; HPRP8; PRPC8; SNRNP220
Function
Plays role in pre-mRNA splicing as core component of precatalytic, catalytic and postcatalytic spliceosomal complexes, both of the predominant U2-type spliceosome and the minor U12-type spliceosome (PubMed:10411133, PubMed:11971955, PubMed:28502770, PubMed:28781166, PubMed:28076346, PubMed:29361316, PubMed:30315277, PubMed:29360106, PubMed:29301961, PubMed:30728453, PubMed:30705154).
Functions as a scaffold that mediates the ordered assembly of spliceosomal proteins and snRNAs. Required for the assembly of the U4/U6-U5 tri-snRNP complex, a building block of the spliceosome. Functions as scaffold that positions spliceosomal U2, U5 and U6 snRNAs at splice sites on pre-mRNA substrates, so that splicing can occur. Interacts with both the 5' and the 3' splice site.
Biological Process
Cellular response to lipopolysaccharideIEA:Ensembl
Cellular response to tumor necrosis factorIEA:Ensembl
mRNA processingManual Assertion Based On ExperimentTAS:ProtInc
mRNA splicing, via spliceosomeManual Assertion Based On ExperimentIDA:UniProtKB
RNA splicingManual Assertion Based On ExperimentTAS:UniProtKB
RNA splicing, via transesterification reactionsManual Assertion Based On ExperimentTAS:UniProtKB
Spliceosomal tri-snRNP complex assemblyManual Assertion Based On ExperimentIDA:UniProtKB
Cellular Location
Nucleus
Nucleus speckle
Involvement in disease
Retinitis pigmentosa 13 (RP13):
A retinal dystrophy belonging to the group of pigmentary retinopathies. Retinitis pigmentosa is characterized by retinal pigment deposits visible on fundus examination and primary loss of rod photoreceptor cells followed by secondary loss of cone photoreceptors. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well.
Ask a question We look forward to hearing from you.
0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Loading...
Have you used Mouse Anti-PRPF8 Recombinant Antibody (2G8)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon $30 eGift Card
Submit a review
Loading...
For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Documents

Contact us

  • Tel: (USA)
  • (UK)
  • Fax:
  • Email:

Submit A Review

Go to
Compare