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Mouse Anti-PRPF8 Recombinant Antibody (2834C1a) (CBMAB-0726CQ)

This product is a mouse antibody that recognizes PRPF8. The antibody 2834C1a can be used for immunoassay techniques such as: Dot, FC, IHC-P, WB.
See all PRPF8 antibodies
Published Data
Fig.1 Western blotting of SF3B1, RBMX, PRPF8 and RNF8 in U2OS cells treated for 48 h with the siRNAs indicated. β-Actin was used as a loading control.
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Summary

Host Animal
Mouse
Specificity
Human, Mouse
Clone
2834C1a
Antibody Isotype
IgG1
Application
Dot, FC, IHC-P, WB

Basic Information

Immunogen
Human recombinant fragment
Specificity
Human, Mouse
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity
>95% as determined by analysis by SDS-PAGE
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Pre-mRNA processing factor 8
Introduction
Pre-mRNA splicing occurs in 2 sequential transesterification steps. Functions as a scaffold that mediates the ordered assembly of spliceosomal proteins and snRNAs. Required for the assembly of the U4/U6-U5 tri-snRNP complex. Functions as scaffold that positions spliceosomal U2, U5 and U6 snRNAs at splice sites on pre-mRNA substrates, so that splicing can occur. Interacts with both the 5 and the 3 splice site.
Entrez Gene ID
Human10594
Mouse192159
UniProt ID
HumanQ6P2Q9
MouseQ99PV0
Alternative Names
PRP8; RP13; HPRP8; PRPC8; SNRNP220
Function
Plays role in pre-mRNA splicing as core component of precatalytic, catalytic and postcatalytic spliceosomal complexes, both of the predominant U2-type spliceosome and the minor U12-type spliceosome (PubMed:10411133, PubMed:11971955, PubMed:28502770, PubMed:28781166, PubMed:28076346, PubMed:29361316, PubMed:30315277, PubMed:29360106, PubMed:29301961, PubMed:30728453, PubMed:30705154).
Functions as a scaffold that mediates the ordered assembly of spliceosomal proteins and snRNAs. Required for the assembly of the U4/U6-U5 tri-snRNP complex, a building block of the spliceosome. Functions as scaffold that positions spliceosomal U2, U5 and U6 snRNAs at splice sites on pre-mRNA substrates, so that splicing can occur. Interacts with both the 5' and the 3' splice site.
Biological Process
Cellular response to lipopolysaccharideIEA:Ensembl
Cellular response to tumor necrosis factorIEA:Ensembl
mRNA processingManual Assertion Based On ExperimentTAS:ProtInc
mRNA splicing, via spliceosomeManual Assertion Based On ExperimentIDA:UniProtKB
RNA splicingManual Assertion Based On ExperimentTAS:UniProtKB
RNA splicing, via transesterification reactionsManual Assertion Based On ExperimentTAS:UniProtKB
Spliceosomal tri-snRNP complex assemblyManual Assertion Based On ExperimentIDA:UniProtKB
Cellular Location
Nucleus
Nucleus speckle
Involvement in disease
Retinitis pigmentosa 13 (RP13):
A retinal dystrophy belonging to the group of pigmentary retinopathies. Retinitis pigmentosa is characterized by retinal pigment deposits visible on fundus examination and primary loss of rod photoreceptor cells followed by secondary loss of cone photoreceptors. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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