Mouse Anti-PRTN3 Recombinant Antibody (CBT1060) (V2LY-0625-LY3069)
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Basic Information
| Application | Note |
| ELISA | 1:10,000 |
| WB | 1:500-1:2,000 |
Formulations & Storage [For reference only, actual COA shall prevail!]
Target
By cleaving and activating receptor F2RL1/PAR-2, enhances endothelial cell barrier function and thus vascular integrity during neutrophil transendothelial migration (PubMed:23202369).
May play a role in neutrophil transendothelial migration, probably when associated with CD177 (PubMed:22266279).
Cell-cell junction maintenanceManual Assertion Based On ExperimentIMP:UniProtKB
Collagen catabolic processIEA:UniProtKB-KW
Mature conventional dendritic cell differentiationManual Assertion Based On ExperimentIDA:UniProtKB
Membrane protein ectodomain proteolysisManual Assertion Based On ExperimentIMP:UniProtKB
Negative regulation of phagocytosisManual Assertion Based On ExperimentIDA:UniProtKB
Neutrophil extravasationManual Assertion Based On ExperimentIMP:UniProtKB
PhagocytosisManual Assertion Based On ExperimentIBA:GO_Central
Positive regulation of cell population proliferationManual Assertion Based On ExperimentTAS:ProtInc
Positive regulation of GTPase activityManual Assertion Based On ExperimentIMP:UniProtKB
ProteolysisManual Assertion Based On ExperimentIDA:UniProtKB
Secreted
Cell membrane
Membrane raft
Localizes predominantly to azurophil granules (primary secretory granules) in neutrophils (PubMed:2033050, PubMed:3198760, PubMed:7897245, PubMed:18462208).
Secreted upon neutrophil stimulation by TNF-alpha, lipopolysaccharide (LPS), fMLP and CXCL8/IL8 or during neutrophil transmigration (PubMed:22266279, PubMed:28240246).
Following secretion tethered to the cell membrane by CD177 (PubMed:18462208, PubMed:22266279).
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Please try the standard protocols which include: protocols, troubleshooting and guide.
Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols
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Custom Antibody Labeling
We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).
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