Search :
Sign in or Register  
Welcome Sign in or Don't have an account?Register

Mouse Anti-PSMB8 Recombinant Antibody (1B3) (CBMAB-P3188-YC)


See all PSMB8 antibodies
Online Inquiry

Summary

Host Animal
Mouse
Specificity
Human
Clone
1B3
Antibody Isotype
IgG2a, κ
Application
ELISA, IF, IHC-P, IP, WB

Basic Information

Immunogen
PSMB8 (AAH01114, 173-272 aa) partial recombinant protein with GST tag. Immunogen sequence: DKKGPGLYYV DEHGTRLSGN MFSTGSGNTY AYGVMDSGYR PNLSPEEAYD LGRRAIAYAT HRDSYSGGVV NMYHMKEDGW VKVESTDVSD LLHQYREANQ
Specificity
Human
Antibody Isotype
IgG2a, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.
Epitope
aa 173-272

Target

Full Name
proteasome (prosome, macropain) subunit, beta type, 8 (large multifunctional peptidase 7)
Introduction
PSMB8 (Proteasome Subunit Beta 8) is a protein coding gene. Diseases associated with PSMB8 include Autoinflammation, Lipodystrophy, And Dermatosis Syndrome and Nakajo Syndrome. Among its related pathways are Cell Cycle, Mitotic and RET signaling. Gene Ontology annotations related to this gene include threonine-type endopeptidase activity. An important paralog of the gene is PSMB5.
Entrez Gene ID
5696
UniProt ID
P28062
Alternative Names
D6S216E; D6S216; PSMB5i; NKJO; LMP7; ALDD; D6S216; D6S216E; JMP; LMP7; NKJO; PSMB5i; RING10; RING10; ALDD; JMP
Function
The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This subunit is involved in antigen processing to generate class I binding peptides. Replacement of PSMB5 by PSMB8 increases the capacity of the immunoproteasome to cleave model peptides after hydrophobic and basic residues. Involved in the generation of spliced peptides resulting from the ligation of two separate proteasomal cleavage products that are not contiguous in the parental protein (PubMed:27049119).
Acts as a major component of interferon gamma-induced sensitivity. Plays a key role in apoptosis via the degradation of the apoptotic inhibitor MCL1. May be involved in the inflammatory response pathway. In cancer cells, substitution of isoform 1 (E2) by isoform 2 (E1) results in immunoproteasome deficiency. Required for the differentiation of preadipocytes into adipocytes.
Biological Process
Antigen processing and presentationIEA:Ensembl
Fat cell differentiationManual Assertion Based On ExperimentIMP:UniProtKB
Proteasomal protein catabolic processManual Assertion Based On ExperimentIBA:GO_Central
Regulation of endopeptidase activityManual Assertion Based On ExperimentIMP:UniProtKB
Cellular Location
Cytoplasm
Nucleus
Involvement in disease
Proteasome-associated autoinflammatory syndrome 1 (PRAAS1):
An autosomal recessive autoinflammatory disorder characterized by early childhood onset of recurrent fever, joint stiffness and severe contractures of the hands and feet, and erythematous skin lesions with subsequent development of lipodystrophy and laboratory evidence of immune dysregulation. Accompanying features may include muscle weakness and atrophy, hepatosplenomegaly, and microcytic anemia.
PTM
Autocleaved. The resulting N-terminal Thr residue of the mature subunit is responsible for the nucleophile proteolytic activity.
More Infomation
Ask a question We look forward to hearing from you.
0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Loading...
Have you used Mouse Anti-PSMB8 Recombinant Antibody (1B3)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon $30 eGift Card
Submit a review
Loading...
For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Learn more

Documents

Request bulk or custom quote Online Inquiry
Second antibody and isotype control

Contact us

  • Tel: (USA)
  • (UK)
  • Fax:
  • Email:

Submit A Review

Online Inquiry