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Mouse Anti-PSMB8 Recombinant Antibody (832913) (CBMAB-L2627-YJ)


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Summary

Host Animal
Mouse
Specificity
Human
Clone
832913
Antibody Isotype
IgG2b
Application
WB

Basic Information

Immunogen
E. coli-derived recombinant human LMP7/PSMB8, Thr73-Gln276, Accession # P28062.
Specificity
Human
Antibody Isotype
IgG2b
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Buffer
PBS, Trehalose
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
proteasome (prosome, macropain) subunit, beta type, 8 (large multifunctional peptidase 7)
Introduction
The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This subunit is involved in antigen processing to generate class I binding peptides. Replacement of PSMB5 by PSMB8 increases the capacity of the immunoproteasome to cleave model peptides after hydrophobic and basic residues. Acts as a major component of interferon gamma-induced sensitivity. PSMB8 plays a key role in apoptosis via the degradation of the apoptotic inhibitor MCL1. PSMB8 may be involved in the inflammatory response pathway. In cancer cells, substitution of isoform 1 (E2) by isoform 2 (E1) results in immunoproteasome deficiency. It is required for the differentiation of preadipocytes into adipocytes.
Entrez Gene ID
5696
UniProt ID
P28062
Alternative Names
JMP; ALDD; LMP7; NKJO; D6S216; PRAAS1; PSMB5i; RING10; D6S216E
Function
The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This subunit is involved in antigen processing to generate class I binding peptides. Replacement of PSMB5 by PSMB8 increases the capacity of the immunoproteasome to cleave model peptides after hydrophobic and basic residues. Involved in the generation of spliced peptides resulting from the ligation of two separate proteasomal cleavage products that are not contiguous in the parental protein (PubMed:27049119).
Acts as a major component of interferon gamma-induced sensitivity. Plays a key role in apoptosis via the degradation of the apoptotic inhibitor MCL1. May be involved in the inflammatory response pathway. In cancer cells, substitution of isoform 1 (E2) by isoform 2 (E1) results in immunoproteasome deficiency. Required for the differentiation of preadipocytes into adipocytes.
Biological Process
Antigen processing and presentationIEA:Ensembl
Fat cell differentiationManual Assertion Based On ExperimentIMP:UniProtKB
Proteasomal protein catabolic processManual Assertion Based On ExperimentIBA:GO_Central
Regulation of endopeptidase activityManual Assertion Based On ExperimentIMP:UniProtKB
Cellular Location
Cytoplasm
Nucleus
Involvement in disease
Proteasome-associated autoinflammatory syndrome 1 (PRAAS1):
An autosomal recessive autoinflammatory disorder characterized by early childhood onset of recurrent fever, joint stiffness and severe contractures of the hands and feet, and erythematous skin lesions with subsequent development of lipodystrophy and laboratory evidence of immune dysregulation. Accompanying features may include muscle weakness and atrophy, hepatosplenomegaly, and microcytic anemia.
PTM
Autocleaved. The resulting N-terminal Thr residue of the mature subunit is responsible for the nucleophile proteolytic activity.
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For research use only. Not intended for any clinical use.

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