Rabbit Anti-PSME1 Recombinant Antibody (
29D5) (V2LY-0725-LY1507)

Name: Rabbit Anti-PSME1 Recombinant Antibody (29D5)
SKU: V2LY-0725-LY1507
Rating: 5 (1 reviews)

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Tested Data

Rabbit Anti-PSME1 Recombinant Antibody (29D5) (V2LY-0725-LY1507) in WB

Western Blot
Positive WB detected in: Jurkat whole cell lysate, HepG2 whole cell lysate, MCF-7 whole cell lysate, A549 whole cell lysate, PC3 whole cell lysate, Hela whole cell lysate, Ntera-2 whole cell lysate, U251 whole cell lysate
All lanes: PSME1 antibody at 1:2000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 29, 27 kDa
Observed band size: 28-30, 55-72 kDa

Rabbit Anti-PSME1 Recombinant Antibody (29D5) (V2LY-0725-LY1507) in IHC

IHC image of V2LY-0725-LY1507 diluted at 1:100 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.

Rabbit Anti-PSME1 Recombinant Antibody (29D5) (V2LY-0725-LY1507) in IHC

IHC image of V2LY-0725-LY1507 diluted at 1:100 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.

Rabbit Anti-PSME1 Recombinant Antibody (29D5) (V2LY-0725-LY1507) in IF

Immunofluorescence staining of HepG2 cell with V2LY-0725-LY1507 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 518-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Rabbit Anti-PSME1 Recombinant Antibody (29D5) (V2LY-0725-LY1507) in FC

Overlay Peak curve showing HepG2 cells stained with V2LY-0725-LY1507 (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4°C.Control antibody (green line) was rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of>10,000 events was performed.

Datasheet

SDS

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Basic Information

Host Animal

Rabbit

Clone

29D5

Application

ELISA, WB, IHC, IF, FC

Immunogen

A synthesized peptide derived from human PSME1.

Host Species

Rabbit

Specificity

Human

Antibody Isotype

IgG

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:500-1:2,000
IHC	1:50-1:200
IF	1:50-1:200
FC	1:50-1:200

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, glycerol

Preservative

Sodium azide

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Proteasome Activator Subunit 1

Entrez Gene ID

5720

UniProt ID

Q06323

Function

Implicated in immunoproteasome assembly and required for efficient antigen processing. The PA28 activator complex enhances the generation of class I binding peptides by altering the cleavage pattern of the proteasome.

Biological Process

Antigen processing and presentation of exogenous antigenIEA:Ensembl
Positive regulation of endopeptidase activityManual Assertion Based On ExperimentIBA:GO_Central
Regulation of G1/S transition of mitotic cell cycleManual Assertion Based On ExperimentIBA:GO_Central
Regulation of proteasomal protein catabolic processManual Assertion Based On ExperimentIBA:GO_Central

Cellular Location

cytoplasm
cytosol
extracellular exosome
nucleoplasm
proteasome activator complex
proteasome complex

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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