Search :
Sign in or Register  
Welcome Sign in or Don't have an account?Register

Mouse Anti-PSME3 Recombinant Antibody (CBT983) (V2LY-0625-LY3125)


See all PSME3 antibodies
Online Inquiry

Summary

Host Animal
Mouse
Specificity
Human, Mouse, Dog, Pig
Clone
CBT983
Antibody Isotype
IgG
Application
WB, IF, ICC

Basic Information

Immunogen
Purified recombinant human PA28γ protein fragments expressed in E.coli.
Host Species
Mouse
Specificity
Human, Mouse, Dog, Pig
Antibody Isotype
IgG
Clonality
Monoclonal Antibody
Application Notes
ApplicationNote
IF1:100-1:500
WB1:1,000-1:2,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
Tris-Glycine, Glycerol
Preservative
Sodium azide
Concentration
Batch dependent
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
Proteasome Activator Subunit 3
Entrez Gene ID
10197
UniProt ID
P61289
Function
Subunit of the 11S REG-gamma (also called PA28-gamma) proteasome regulator, a doughnut-shaped homoheptamer which associates with the proteasome. 11S REG-gamma activates the trypsin-like catalytic subunit of the proteasome but inhibits the chymotrypsin-like and postglutamyl-preferring (PGPH) subunits. Facilitates the MDM2-p53/TP53 interaction which promotes ubiquitination- and MDM2-dependent proteasomal degradation of p53/TP53, limiting its accumulation and resulting in inhibited apoptosis after DNA damage. May also be involved in cell cycle regulation. Mediates CCAR2 and CHEK2-dependent SIRT1 inhibition (PubMed:25361978).
Biological Process
Apoptotic processIEA:UniProtKB-KW
Cell cycleIEA:UniProtKB-KW
Negative regulation of extrinsic apoptotic signaling pathwayManual Assertion Based On ExperimentIDA:UniProtKB
Positive regulation of endopeptidase activityManual Assertion Based On ExperimentIBA:GO_Central
Regulation of G1/S transition of mitotic cell cycleManual Assertion Based On ExperimentIBA:GO_Central
Regulation of proteasomal protein catabolic processManual Assertion Based On ExperimentIDA:UniProtKB
Cellular Location
Nucleus
Cytoplasm
Localizes to the cytoplasm during mitosis following nuclear envelope breakdown at this distinct stage of the cell cycle which allows its interaction with MAP3K3 kinase.
PTM
Phosphorylated by MAP3K3 (By similarity).
Phosphorylation at Ser-247 promotes its association with CCAR2.
Acetylation at the major site Lys-195 is important for oligomerization and ability to degrade its target substrates. Deacetylated by SIRT1.
More Infomation
Ask a question We look forward to hearing from you.
0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Loading...
Have you used Mouse Anti-PSME3 Recombinant Antibody (CBT983)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon $30 eGift Card
Submit a review
Loading...
For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Learn more

Documents

Request bulk or custom quote
Second antibody and isotype control

Contact us

  • Tel: (USA)
  • (UK)
  • Fax:
  • Email:

Submit A Review

Online Inquiry