Mouse Anti-PTBP1 Recombinant Antibody (3H12) (CBMAB-BR407LY)

Name: Mouse Anti-PTBP1 Recombinant Antibody (3H12)
SKU: CBMAB-BR407LY
Rating: 5 (1 reviews)

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Datasheet

SDS

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Basic Information

Host Animal

Mouse

Clone

3H12

Application

FC: 1-3 μg/1x10 cells, IF: 1:1000 dilution, IHC-P: 0.5-1 μg/ml, ICC: 1:200 dilution, WB: 0.1-0.5 μg/ml

Immunogen

E.coli-derived human PTBP1 recombinant protein (Position: M1-A504).

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG2b

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Lyophilized

Preservative

0.05 mg sodium azide

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Polypyrimidine tract-binding protein 1

Entrez Gene ID

Human	5725
Mouse	19205
Rat	29497

UniProt ID

Human	P26599
Mouse	P17225
Rat	Q00438

Alternative Names

Polypyrimidine tract-binding protein 1; PTB; 57 kDa RNA-binding protein PPTB-1; Heterogeneous nuclear ribonucleoprotein I; hnRNP I; PTBP1; PTB

Function

Plays a role in pre-mRNA splicing and in the regulation of alternative splicing events. Activates exon skipping of its own pre-mRNA during muscle cell differentiation. Binds to the polypyrimidine tract of introns. May promote RNA looping when bound to two separate polypyrimidine tracts in the same pre-mRNA. May promote the binding of U2 snRNP to pre-mRNA. Cooperates with RAVER1 to modulate switching between mutually exclusive exons during maturation of the TPM1 pre-mRNA. Represses the splicing of MAPT/Tau exon 10 (PubMed:15009664).
Binds to polypyrimidine-rich controlling element (PCE) of CFTR and promotes exon skipping of CFTR exon 9, thereby antagonizing TIA1 and its role in exon inclusion of CFTR exon 9 (PubMed:14966131).
Plays a role in the splicing of pyruvate kinase PKM by binding repressively to a polypyrimidine tract flanking PKM exon 9, inhibiting exon 9 inclusion and resulting in exon 10 inclusion and production of the PKM M2 isoform (PubMed:20010808).
In case of infection by picornaviruses, binds to the viral internal ribosome entry site (IRES) and stimulates the IRES-mediated translation (PubMed:21518806).

Biological Process

IRES-dependent viral translational initiationManual Assertion Based On ExperimentIDA:FlyBase
mRNA processingManual Assertion Based On ExperimentTAS:ProtInc
Negative regulation of mRNA splicing, via spliceosomeManual Assertion Based On ExperimentIDA:UniProtKB
Negative regulation of muscle cell differentiationManual Assertion Based On ExperimentIDA:UniProtKB
Negative regulation of RNA splicingManual Assertion Based On ExperimentIDA:UniProtKB
Positive regulation of calcineurin-NFAT signaling cascadeManual Assertion Based On ExperimentIMP:BHF-UCL
Positive regulation of protein dephosphorylationManual Assertion Based On ExperimentIMP:BHF-UCL
Regulation of alternative mRNA splicing, via spliceosomeManual Assertion Based On ExperimentIDA:UniProtKB
Regulation of cell differentiationManual Assertion Based On ExperimentIBA:GO_Central
Regulation of RNA splicingManual Assertion Based On ExperimentIBA:GO_Central
RNA splicingManual Assertion Based On ExperimentTAS:ProtInc

Cellular Location

Nucleus

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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