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Mouse Anti-PTBP1 Recombinant Antibody (3H12) (CBMAB-BR407LY)

The product is antibody recognizes PTBP1. The antibody 3H12 immunoassay techniques such as: FC, IF, IHC-P, ICC, WB.
See all PTBP1 antibodies

Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
3H12
Antibody Isotype
IgG2b
Application
FC: 1-3 μg/1x10 cells, IF: 1:1000 dilution, IHC-P: 0.5-1 μg/ml, ICC: 1:200 dilution, WB: 0.1-0.5 μg/ml

Basic Information

Immunogen
E.coli-derived human PTBP1 recombinant protein (Position: M1-A504).
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG2b
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Lyophilized
Preservative
0.05 mg sodium azide
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
Polypyrimidine tract-binding protein 1
Entrez Gene ID
Human5725
Mouse19205
Rat29497
UniProt ID
HumanP26599
MouseP17225
RatQ00438
Alternative Names
Polypyrimidine tract-binding protein 1; PTB; 57 kDa RNA-binding protein PPTB-1; Heterogeneous nuclear ribonucleoprotein I; hnRNP I; PTBP1; PTB
Function
Plays a role in pre-mRNA splicing and in the regulation of alternative splicing events. Activates exon skipping of its own pre-mRNA during muscle cell differentiation. Binds to the polypyrimidine tract of introns. May promote RNA looping when bound to two separate polypyrimidine tracts in the same pre-mRNA. May promote the binding of U2 snRNP to pre-mRNA. Cooperates with RAVER1 to modulate switching between mutually exclusive exons during maturation of the TPM1 pre-mRNA. Represses the splicing of MAPT/Tau exon 10 (PubMed:15009664).
Binds to polypyrimidine-rich controlling element (PCE) of CFTR and promotes exon skipping of CFTR exon 9, thereby antagonizing TIA1 and its role in exon inclusion of CFTR exon 9 (PubMed:14966131).
Plays a role in the splicing of pyruvate kinase PKM by binding repressively to a polypyrimidine tract flanking PKM exon 9, inhibiting exon 9 inclusion and resulting in exon 10 inclusion and production of the PKM M2 isoform (PubMed:20010808).
In case of infection by picornaviruses, binds to the viral internal ribosome entry site (IRES) and stimulates the IRES-mediated translation (PubMed:21518806).
Biological Process
IRES-dependent viral translational initiationManual Assertion Based On ExperimentIDA:FlyBase
mRNA processingManual Assertion Based On ExperimentTAS:ProtInc
Negative regulation of mRNA splicing, via spliceosomeManual Assertion Based On ExperimentIDA:UniProtKB
Negative regulation of muscle cell differentiationManual Assertion Based On ExperimentIDA:UniProtKB
Negative regulation of RNA splicingManual Assertion Based On ExperimentIDA:UniProtKB
Positive regulation of calcineurin-NFAT signaling cascadeManual Assertion Based On ExperimentIMP:BHF-UCL
Positive regulation of protein dephosphorylationManual Assertion Based On ExperimentIMP:BHF-UCL
Regulation of alternative mRNA splicing, via spliceosomeManual Assertion Based On ExperimentIDA:UniProtKB
Regulation of cell differentiationManual Assertion Based On ExperimentIBA:GO_Central
Regulation of RNA splicingManual Assertion Based On ExperimentIBA:GO_Central
RNA splicingManual Assertion Based On ExperimentTAS:ProtInc
Cellular Location
Nucleus
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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