Rabbit Anti-PTBP1 Recombinant Antibody (
8A1) (V2LY-0725-LY1575)

Name: Rabbit Anti-PTBP1 Recombinant Antibody (8A1)
SKU: V2LY-0725-LY1575
Rating: 5 (1 reviews)

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Tested Data

Rabbit Anti-PTBP1 Recombinant Antibody (8A1) (V2LY-0725-LY1575) in WB

Western Blot
Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate, PC-3 whole cell lysate, K562 whole cell lysate, HL-60 whole cell lysate, MCF-7 whole cell lysate, Jurkat whole cell lysate
All lanes: PTBP1 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 58, 60, 60 kDa
Observed band size: 58 kDa

Rabbit Anti-PTBP1 Recombinant Antibody (8A1) (V2LY-0725-LY1575) in IHC

IHC image of V2LY-0725-LY1575 diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

Rabbit Anti-PTBP1 Recombinant Antibody (8A1) (V2LY-0725-LY1575) in IHC

IHC image of V2LY-0725-LY1575 diluted at 1:100 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

Datasheet

SDS

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Basic Information

Host Animal

Rabbit

Clone

8A1

Application

ELISA, WB, IHC

Immunogen

A synthesized peptide derived from human PTBP1.

Host Species

Rabbit

Specificity

Human

Antibody Isotype

IgG

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:500-1:5,000
IHC	1:50-1:200

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, glycerol

Preservative

Sodium azide

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Polypyrimidine tract-binding protein 1

Entrez Gene ID

5725

UniProt ID

P26599

Function

Plays a role in pre-mRNA splicing and in the regulation of alternative splicing events. Activates exon skipping of its own pre-mRNA during muscle cell differentiation. Binds to the polypyrimidine tract of introns. May promote RNA looping when bound to two separate polypyrimidine tracts in the same pre-mRNA. May promote the binding of U2 snRNP to pre-mRNA. Cooperates with RAVER1 to modulate switching between mutually exclusive exons during maturation of the TPM1 pre-mRNA. Represses the splicing of MAPT/Tau exon 10 (PubMed:15009664).
Binds to polypyrimidine-rich controlling element (PCE) of CFTR and promotes exon skipping of CFTR exon 9, thereby antagonizing TIA1 and its role in exon inclusion of CFTR exon 9 (PubMed:14966131).
Plays a role in the splicing of pyruvate kinase PKM by binding repressively to a polypyrimidine tract flanking PKM exon 9, inhibiting exon 9 inclusion and resulting in exon 10 inclusion and production of the PKM M2 isoform (PubMed:20010808).
In case of infection by picornaviruses, binds to the viral internal ribosome entry site (IRES) and stimulates the IRES-mediated translation (PubMed:21518806).

Biological Process

IRES-dependent viral translational initiationManual Assertion Based On ExperimentIDA:FlyBase
mRNA processingManual Assertion Based On ExperimentTAS:ProtInc
Negative regulation of mRNA splicing, via spliceosomeManual Assertion Based On ExperimentIDA:UniProtKB
Negative regulation of muscle cell differentiationManual Assertion Based On ExperimentIDA:UniProtKB
Negative regulation of RNA splicingManual Assertion Based On ExperimentIDA:UniProtKB
Positive regulation of calcineurin-NFAT signaling cascadeManual Assertion Based On ExperimentIMP:BHF-UCL
Positive regulation of protein dephosphorylationManual Assertion Based On ExperimentIMP:BHF-UCL
Regulation of alternative mRNA splicing, via spliceosomeManual Assertion Based On ExperimentIDA:UniProtKB
Regulation of cell differentiationManual Assertion Based On ExperimentIBA:GO_Central
Regulation of RNA splicingManual Assertion Based On ExperimentIBA:GO_Central
RNA splicingManual Assertion Based On ExperimentTAS:ProtInc

Cellular Location

Nucleus

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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