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Mouse Anti-PTTG1 Recombinant Antibody (1D9) (CBMAB-P3503-YC)

Provided herein is a Mouse monoclonal antibody against Human Pituitary tumor-transforming 1. The antibody can be used for immunoassay techniques, such as ELISA, WB.
See all PTTG1 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
1D9
Antibody Isotype
IgG1, κ
Application
ELISA, WB

Basic Information

Immunogen
PTTG1 (NP_004210, 1-100 aa) partial recombinant protein with GST tag. Immunogen sequence: MATLIYVDKE NGEPGTRVVA KDGLKLGSGP SIKALDGRSQ VSTPRFGKTF DAPPALPKAT RKALGTVNRA TEKSVKTKGP LKQKQPSFSA KKMTEKTVKA
Specificity
Human
Antibody Isotype
IgG1, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
pituitary tumor-transforming 1
Introduction
PTTG1 is a homolog of yeast securin proteins, which prevent separins from promoting sister chromatid separation. It is an anaphase-promoting complex (APC) substrate that associates with a separin until activation of the APC. The gene product has transforming activity in vitro and tumorigenic activity in vivo, and the gene is highly expressed in various tumors. The gene product contains 2 PXXP motifs, which are required for its transforming and tumorigenic activities, as well as for its stimulation of basic fibroblast growth factor expression. It also contains a destruction box (D box) that is required for its degradation by the APC. The acidic C-terminal region of the encoded protein can act as a transactivation domain. The gene product is mainly a cytosolic protein, although it partially localizes in the nucleus.
Entrez Gene ID
9232
UniProt ID
O95997
Alternative Names
PTTG; AW555095; TUTR1; C87862; Pttg; HPTTG; Pttg3; EAP1
Function
Regulatory protein, which plays a central role in chromosome stability, in the p53/TP53 pathway, and DNA repair. Probably acts by blocking the action of key proteins. During the mitosis, it blocks Separase/ESPL1 function, preventing the proteolysis of the cohesin complex and the subsequent segregation of the chromosomes. At the onset of anaphase, it is ubiquitinated, conducting to its destruction and to the liberation of ESPL1. Its function is however not limited to a blocking activity, since it is required to activate ESPL1. Negatively regulates the transcriptional activity and related apoptosis activity of TP53. The negative regulation of TP53 may explain the strong transforming capability of the protein when it is overexpressed. May also play a role in DNA repair via its interaction with Ku, possibly by connecting DNA damage-response pathways with sister chromatid separation.
Biological Process
Cell divisionIEA:UniProtKB-KW
Chromosome organizationIEA:InterPro
DNA repairIEA:UniProtKB-KW
Homologous chromosome segregationManual Assertion Based On ExperimentIBA:GO_Central
Negative regulation of mitotic sister chromatid separationManual Assertion Based On ExperimentIBA:GO_Central
SpermatogenesisManual Assertion Based On ExperimentTAS:ProtInc
Cellular Location
Cytoplasm
Nucleus
PTM
Phosphorylated at Ser-165 by CDK1 during mitosis.
Phosphorylated in vitro by ds-DNA kinase.
Ubiquitinated through 'Lys-11' linkage of ubiquitin moieties by the anaphase promoting complex (APC) at the onset of anaphase, conducting to its degradation. 'Lys-11'-linked ubiquitination is mediated by the E2 ligase UBE2C/UBCH10.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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