Mouse Anti-RBBP8 Recombinant Antibody (19E8) (CBMAB-R1657-CN)

Name: Mouse Anti-RBBP8 Recombinant Antibody (19E8)
SKU: CBMAB-R1657-CN
Rating: 5 (1 reviews)

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Datasheet

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Basic Information

Host Animal

Mouse

Clone

19E8

Application

Specificity

Human

Antibody Isotype

IgG

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Buffer

PBS

Concentration

1.3 mg/mL

More Infomation

Target

Full Name

RB Binding Protein 8, Endonuclease

Introduction

The protein encoded by this gene is a ubiquitously expressed nuclear protein. It is found among several proteins that bind directly to retinoblastoma protein, which regulates cell proliferation. This protein complexes with transcriptional co-repressor CTBP. It is also associated with BRCA1 and is thought to modulate the functions of BRCA1 in transcriptional regulation, DNA repair, and/or cell cycle checkpoint control. It is suggested that this gene may itself be a tumor suppressor acting in the same pathway as BRCA1. Three transcript variants encoding two different isoforms have been found for this gene. More transcript variants exist, but their full-length natures have not been determined. [provided by RefSeq, Jul 2008]

Entrez Gene ID

5932

UniProt ID

Q99708

Alternative Names

RB Binding Protein 8, Endonuclease; CTBP-Interacting Protein; Sporulation In The Absence Of SPO11 Protein 2 Homolog; Retinoblastoma-Binding Protein 8; Retinoblastoma Binding Protein 8; RBBP-8; CTIP; SAE2; RIM;

Function

Endonuclease that cooperates with the MRE11-RAD50-NBN (MRN) complex in DNA-end resection, the first step of double-strand break (DSB) repair through the homologous recombination (HR) pathway (PubMed:17965729, PubMed:19202191, PubMed:19759395, PubMed:20064462, PubMed:26721387).
HR is restricted to S and G2 phases of the cell cycle and preferentially repairs DSBs resulting from replication fork collapse (PubMed:17965729, PubMed:19202191).
Key determinant of DSB repair pathway choice, as it commits cells to HR by preventing classical non-homologous end-joining (NHEJ) (PubMed:19202191).
Functions downstream of the MRN complex and ATM, promotes ATR activation and its recruitment to DSBs in the S/G2 phase facilitating the generation of ssDNA (PubMed:16581787, PubMed:17965729, PubMed:19759395, PubMed:20064462).
Component of the BRCA1-RBBP8 complex that regulates CHEK1 activation and controls cell cycle G2/M checkpoints on DNA damage (PubMed:15485915, PubMed:16818604).
During immunoglobulin heavy chain class-switch recombination, promotes microhomology-mediated alternative end joining (A-NHEJ) and plays an essential role in chromosomal translocations (By similarity).

Biological Process

Biological Process blastocyst hatchingIEA:Ensembl
Biological Process cell divisionIEA:UniProtKB-KW
Biological Process DNA double-strand break processing involved in repair via single-strand annealingManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process DNA repairManual Assertion Based On ExperimentTAS:ProtInc
Biological Process DNA strand resection involved in replication fork processing1 PublicationIC:ComplexPortal
Biological Process double-strand break repair via homologous recombinationManual Assertion Based On ExperimentIBA:GO_Central
Biological Process G1/S transition of mitotic cell cycleIEA:Ensembl
Biological Process homologous recombination1 PublicationIC:ComplexPortal
Biological Process meiotic cell cycleIEA:UniProtKB-KW
Biological Process mitotic G2/M transition checkpoint1 PublicationIC:ComplexPortal
Biological Process positive regulation of double-strand break repair via homologous recombinationManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process regulation of transcription by RNA polymerase IIManual Assertion Based On ExperimentTAS:ProtInc

Cellular Location

Nucleus
Chromosome
Associates with sites of DNA damage in S/G2 phase (PubMed:10764811, PubMed:25349192).
Ubiquitinated RBBP8 binds to chromatin following DNA damage (PubMed:16818604).

Involvement in disease

Seckel syndrome 2 (SCKL2):
A rare autosomal recessive disorder characterized by proportionate dwarfism of prenatal onset associated with low birth weight, growth retardation, severe microcephaly with a bird-headed like appearance, and intellectual disability.
Jawad syndrome (JWDS):
A syndrome characterized by congenital microcephaly, moderately severe intellectual disability, and symmetrical digital anomalies. Digital malformations of variable degree include hallux valgus, syndactyly of toes 4 and 5, short fifth fingers, single flexion crease of fifth fingers, polydactyly and synpolydactyly.

PTM

Hyperphosphorylation upon ionizing radiation results in dissociation from BRCA1. Phosphorylation at Thr-847 by CDK1 is essential for the recruitment to DNA and the DNA repair function. Phosphorylated on Ser-327 as cells enter G2 phase. This phosphorylation is required for binding BRCA1 and for the G2/M DNA damage transition checkpoint control. Phosphorylation at Thr-315, probably catalyzed by CDK2, is required for PIN1-binding, while phosphorylation at Ser-276 serves as a PIN1 isomerization site. Phosphorylation at Thr-315 is cell-cycle dependent. It steadily increases during S phase, peaks at late S/G2 phase, and drops at G1 (PubMed:23623683).
Ubiquitinated (PubMed:14654780, PubMed:16818604, PubMed:27561354).
Ubiquitination at multiple sites by BRCA1 (via its N-terminal RING domain) does not lead to its proteasomal degradation but instead the ubiquitinated RBBP8 binds to chromatin following DNA damage and may play a role in G2/M checkpoint control (PubMed:16818604).
Ubiquitinated by RNF138 at its N-terminus (PubMed:26502057).
Ubiquitinated through 'Lys-48' by the E3 CUL3-KLHL15 complex; this modification leads to proteasomal degradation (PubMed:27561354).
Ubiquitinated by the E3 FZR1/APC/C complex; this modification leads to proteasomal degradation (PubMed:25349192).

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
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Isotype control

For research use only. Not intended for any clinical use.

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