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Rabbit Anti-RBBP8 Recombinant Antibody (D76F7) (CBMAB-CP0398-LY)

The product is antibody recognizes RBBP8. The antibody D76F7 immunoassay techniques such as: WB.
See all RBBP8 antibodies

Summary

Host Animal
Rabbit
Specificity
Human, Monkey
Clone
D76F7
Antibody Isotype
IgG
Application
WB

Basic Information

Immunogen
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human CtIP protein.
Specificity
Human, Monkey
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
100 µg/ml BSA, 50% glycerol
Preservative
0.02% sodium azide
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
RB Binding Protein 8, Endonuclease
Introduction
The protein encoded by this gene is a ubiquitously expressed nuclear protein. It is found among several proteins that bind directly to retinoblastoma protein, which regulates cell proliferation. This protein complexes with transcriptional co-repressor CTBP. It is also associated with BRCA1 and is thought to modulate the functions of BRCA1 in transcriptional regulation, DNA repair, and/or cell cycle checkpoint control. It is suggested that this gene may itself be a tumor suppressor acting in the same pathway as BRCA1. Three transcript variants encoding two different isoforms have been found for this gene. More transcript variants exist, but their full-length natures have not been determined. [provided by RefSeq, Jul 2008]
Entrez Gene ID
Human5932
Monkey677718
UniProt ID
HumanQ99708
MonkeyF7DF28
Alternative Names
RB Binding Protein 8; Endonuclease; CTBP-Interacting Protein; Sporulation In The Absence Of SPO11 Protein 2 Homolog; Retinoblastoma-Binding Protein 8; Retinoblastoma Binding Protein 8; RBBP-8; CTIP; SAE2; RIM;
Function
Endonuclease that cooperates with the MRE11-RAD50-NBN (MRN) complex in DNA-end resection, the first step of double-strand break (DSB) repair through the homologous recombination (HR) pathway (PubMed:17965729, PubMed:19202191, PubMed:19759395, PubMed:20064462, PubMed:26721387).
HR is restricted to S and G2 phases of the cell cycle and preferentially repairs DSBs resulting from replication fork collapse (PubMed:17965729, PubMed:19202191).
Key determinant of DSB repair pathway choice, as it commits cells to HR by preventing classical non-homologous end-joining (NHEJ) (PubMed:19202191).
Functions downstream of the MRN complex and ATM, promotes ATR activation and its recruitment to DSBs in the S/G2 phase facilitating the generation of ssDNA (PubMed:16581787, PubMed:17965729, PubMed:19759395, PubMed:20064462).
Component of the BRCA1-RBBP8 complex that regulates CHEK1 activation and controls cell cycle G2/M checkpoints on DNA damage (PubMed:15485915, PubMed:16818604).
During immunoglobulin heavy chain class-switch recombination, promotes microhomology-mediated alternative end joining (A-NHEJ) and plays an essential role in chromosomal translocations (By similarity).
Biological Process
Biological Process blastocyst hatchingIEA:Ensembl
Biological Process cell divisionIEA:UniProtKB-KW
Biological Process DNA double-strand break processing involved in repair via single-strand annealingManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process DNA repairManual Assertion Based On ExperimentTAS:ProtInc
Biological Process DNA strand resection involved in replication fork processing1 PublicationIC:ComplexPortal
Biological Process double-strand break repair via homologous recombinationManual Assertion Based On ExperimentIBA:GO_Central
Biological Process G1/S transition of mitotic cell cycleIEA:Ensembl
Biological Process homologous recombination1 PublicationIC:ComplexPortal
Biological Process meiotic cell cycleIEA:UniProtKB-KW
Biological Process mitotic G2/M transition checkpoint1 PublicationIC:ComplexPortal
Biological Process positive regulation of double-strand break repair via homologous recombinationManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process regulation of transcription by RNA polymerase IIManual Assertion Based On ExperimentTAS:ProtInc
Cellular Location
Nucleus
Chromosome
Associates with sites of DNA damage in S/G2 phase (PubMed:10764811, PubMed:25349192).
Ubiquitinated RBBP8 binds to chromatin following DNA damage (PubMed:16818604).
Involvement in disease
Seckel syndrome 2 (SCKL2):
A rare autosomal recessive disorder characterized by proportionate dwarfism of prenatal onset associated with low birth weight, growth retardation, severe microcephaly with a bird-headed like appearance, and intellectual disability.
Jawad syndrome (JWDS):
A syndrome characterized by congenital microcephaly, moderately severe intellectual disability, and symmetrical digital anomalies. Digital malformations of variable degree include hallux valgus, syndactyly of toes 4 and 5, short fifth fingers, single flexion crease of fifth fingers, polydactyly and synpolydactyly.
PTM
Hyperphosphorylation upon ionizing radiation results in dissociation from BRCA1. Phosphorylation at Thr-847 by CDK1 is essential for the recruitment to DNA and the DNA repair function. Phosphorylated on Ser-327 as cells enter G2 phase. This phosphorylation is required for binding BRCA1 and for the G2/M DNA damage transition checkpoint control. Phosphorylation at Thr-315, probably catalyzed by CDK2, is required for PIN1-binding, while phosphorylation at Ser-276 serves as a PIN1 isomerization site. Phosphorylation at Thr-315 is cell-cycle dependent. It steadily increases during S phase, peaks at late S/G2 phase, and drops at G1 (PubMed:23623683).
Ubiquitinated (PubMed:14654780, PubMed:16818604, PubMed:27561354).
Ubiquitination at multiple sites by BRCA1 (via its N-terminal RING domain) does not lead to its proteasomal degradation but instead the ubiquitinated RBBP8 binds to chromatin following DNA damage and may play a role in G2/M checkpoint control (PubMed:16818604).
Ubiquitinated by RNF138 at its N-terminus (PubMed:26502057).
Ubiquitinated through 'Lys-48' by the E3 CUL3-KLHL15 complex; this modification leads to proteasomal degradation (PubMed:27561354).
Ubiquitinated by the E3 FZR1/APC/C complex; this modification leads to proteasomal degradation (PubMed:25349192).
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For research use only. Not intended for any clinical use.

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