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Mouse Anti-RCHY1 Recombinant Antibody (CBT1034) (V2LY-0625-LY3090)


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Summary

Host Animal
Mouse
Specificity
Human, Rat
Clone
CBT1034
Antibody Isotype
IgG
Application
WB, IHC-P, IF, ICC, FC, ELISA

Basic Information

Immunogen
Purified recombinant fragment of human Pirh2 expressed in E. Coli.
Host Species
Mouse
Specificity
Human, Rat
Antibody Isotype
IgG
Clonality
Monoclonal Antibody
Application Notes
ApplicationNote
FC1:200-1:400
IF1:200-1:1,000
IHC1:200-1:1,000
WB1:500-1:2,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Supernatant
Buffer
BSA, Glycerol
Preservative
Sodium azide
Concentration
Batch dependent
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
Ring Finger And CHY Zinc Finger Domain Containing 1
Entrez Gene ID
25898
Function
Mediates E3-dependent ubiquitination and proteasomal degradation of target proteins, including p53/TP53, P73, HDAC1 and CDKN1B. Preferentially acts on tetrameric p53/TP53. Monoubiquitinates the translesion DNA polymerase POLH. Contributes to the regulation of the cell cycle progression. Increases AR transcription factor activity.
Biological Process
Biological Process error-free translesion synthesisTAS:Reactome
Biological Process positive regulation of proteasomal ubiquitin-dependent protein catabolic processManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process positive regulation of protein ubiquitinationManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process protein autoubiquitinationIDA:UniProtKB
Biological Process protein ubiquitinationManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process ubiquitin-dependent protein catabolic processManual Assertion Based On ExperimentIDA:UniProtKB
Cellular Location
Nucleus
Nucleus speckle
Cytoplasm
PTM
Subject to ubiquitination and proteasomal degradation. Interaction with PLAGL2 or KAT5 enhances protein stability.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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