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Mouse Anti-RNF181 Recombinant Antibody (CBYJL-2334) (CBMAB-L2636-YJ)

Provided herein is a Mouse monoclonal antibody, which binds to Ring Finger Protein 181 (RNF181). The antibody can be used for immunoassay techniques, such as WB.
See all RNF181 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
CBYJL-2334
Antibody Isotype
IgG2a, κ
Application
WB

Basic Information

Immunogen
LOC51255 (NP_057578, 90 a.a. ~ 154 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K.
Specificity
Human
Antibody Isotype
IgG2a, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Buffer
PBS, pH 7.4
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Epitope
aa 90-154

Target

Full Name
Ring Finger Protein 181
Introduction
RNF181 binds the integrin alpha-IIb (ITGA2B; MIM 607759)/beta-3 (ITGB3; MIM 173470) complex and has E3 ubiquitin ligase activity.
Entrez Gene ID
51255
UniProt ID
Q9P0P0
Alternative Names
Ring Finger Protein 181; RING-Type E3 Ubiquitin Transferase RNF181; RING Finger Protein 181; E3 Ubiquitin-Protein Ligase RNF181; EC 2.3.2.27; HSPC238
Function
E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates (PubMed:18331836).
Catalyzes monoubiquitination of 26S proteasome subunit PSMC2/RPT1 (PubMed:24811749).
Biological Process
Biological Process protein autoubiquitinationManual Assertion Based On ExperimentIDA:FlyBase
Biological Process protein ubiquitinationManual Assertion Based On ExperimentIBA:GO_Central
Cellular Location
Cytoplasm
PTM
Autoubiquitinated as part of the enzymatic reaction.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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