Mouse Anti-RNF5 Recombinant Antibody (22B3) (CBMAB-R3044-CN)

Name: Mouse Anti-RNF5 Recombinant Antibody (22B3)
SKU: CBMAB-R3044-CN
Rating: 5 (1 reviews)

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Datasheet

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Basic Information

Host Animal

Mouse

Clone

22B3

Application

Immunogen

Human full length recombinant RNF5

Specificity

Human, Mouse

Antibody Isotype

IgG1

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

More Infomation

Target

Full Name

Ring Finger Protein 5

Introduction

The protein encoded by this gene contains a RING finger, which is a motif known to be involved in protein-protein interactions. This protein is a membrane-bound ubiquitin ligase. It can regulate cell motility by targeting paxillin ubiquitination and altering the distribution and localization of paxillin in cytoplasm and cell focal adhesions. [provided by RefSeq, Jul 2008]

Entrez Gene ID

Human	6048
Mouse	54197

UniProt ID

Human	Q99942
Mouse	O35445

Alternative Names

Ring Finger Protein 5; Ring Finger Protein 5, E3 Ubiquitin Protein Ligase; RING-Type E3 Ubiquitin Transferase RNF5; RING Finger Protein 5; Ram1 Homolog; Protein G16; RMA1;

Function

Membrane-bound E3 ubiquitin-protein ligase that mediates ubiquitination of target proteins (PubMed:11329381, PubMed:12861019, PubMed:16176924, PubMed:19285439, PubMed:19269966).
May function together with E2 ubiquitin-conjugating enzymes UBE2D1/UBCH5A and UBE2D2/UBC4 (PubMed:11329381).
Mediates ubiquitination of PXN/paxillin,thereby regulating cell motility and localization of PXN/paxillin (PubMed:12861019).
Catalyzes ubiquitination of Salmonella type III secreted protein sopA (PubMed:16176924).
Mediates the 'Lys-63'-linked polyubiquitination of JKAMP thereby regulating JKAMP function by decreasing its association with components of the proteasome and ERAD; the ubiquitination appears to involve E2 ubiquitin-conjugating enzyme UBE2N (PubMed:19269966).
Mediates the 'Lys-48'-linked polyubiquitination of STING1 at 'Lys-150' leading to its proteasomal degradation; the ubiquitination occurs in mitochondria after viral transfection and regulates antiviral responses (PubMed:19285439).
Catalyzes ubiquitination and subsequent degradation of ATG4B, thereby inhibiting autophagy (PubMed:23093945).

Biological Process

Biological Process endoplasmic reticulum mannose trimmingTAS:Reactome
Biological Process ER-associated misfolded protein catabolic processManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process ERAD pathwayManual Assertion Based On ExperimentIMP:ParkinsonsUK-UCL
Biological Process negative regulation of autophagyIEA:Ensembl
Biological Process protein catabolic processManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process protein destabilizationIEA:Ensembl
Biological Process protein K48-linked ubiquitinationManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process protein K63-linked ubiquitinationManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process regulation of autophagosome assemblyIEA:Ensembl
Biological Process response to bacteriumIEA:Ensembl
Biological Process transmembrane transportTAS:Reactome
Biological Process ubiquitin-dependent ERAD pathwayManual Assertion Based On ExperimentIGI:ParkinsonsUK-UCL
Biological Process ubiquitin-dependent protein catabolic processManual Assertion Based On ExperimentIBA:GO_Central

Cellular Location

Cell membrane
Mitochondrion membrane
Endoplasmic reticulum membrane
Predominantly located in the plasma membrane, with some localization occurring within cytoplasmic organelles.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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